A sensitive and specific hyperbranched rolling circle amplification assay and test strip for white spot syndrome virus

White spot syndrome virus (WSSV) is a global threat to the prawn industry, and there is no simple method for field-based testing of this virus. We designed a padlock probe and primers to the capsid protein gene VP28 of WSSV, and established a hyperbranched rolling circle amplification (HRCA) assay a...

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Published in:Journal of AOAC International 2014-09, Vol.97 (5), p.1410-1415
Main Authors: Zhao, Yu-Ran, Yin, Wei-Li, Yue, Zhi-Qin, Li, Ba-Fang
Format: Article
Language:English
Subjects:
Animals
Control
Diseases
Distribution
Nucleic Acid Amplification Techniques - methods
Pandalidae - virology
Prevention
Reagent Strips
Sensitivity and Specificity
Shrimps
Virus diseases
White spot syndrome virus 1 - genetics
White spot syndrome virus 1 - isolation & purification
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container_issue 5
container_start_page 1410
container_title Journal of AOAC International
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creator Zhao, Yu-Ran
Yin, Wei-Li
Yue, Zhi-Qin
Li, Ba-Fang
description White spot syndrome virus (WSSV) is a global threat to the prawn industry, and there is no simple method for field-based testing of this virus. We designed a padlock probe and primers to the capsid protein gene VP28 of WSSV, and established a hyperbranched rolling circle amplification (HRCA) assay and a corresponding strip-based test. The assay and the test strip both had similar high accuracy and specificity, and their sensitivity was about 10 copies/μL, which is 100 times higher than conventional PCR. In this study, 68 batches of prawns were tested for WSSV with the HRCA assay and test strip, and the results were compared with the PCR assay. The results indicated that both the assay and test strip had accuracy similar to each other and to the PCR results. However, the assay and strip were more sensitive and user-friendly than PCR. Establishment of this method will provide a rapid detection of WSSV and also a basis for field-based detection of animal disease.
doi_str_mv 10.5740/jaoacint.13-387
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source Oxford Journals Online
subjects Animals
Control
Diseases
Distribution
Nucleic Acid Amplification Techniques - methods
Pandalidae - virology
Prevention
Reagent Strips
Sensitivity and Specificity
Shrimps
Virus diseases
White spot syndrome virus 1 - genetics
White spot syndrome virus 1 - isolation & purification
title A sensitive and specific hyperbranched rolling circle amplification assay and test strip for white spot syndrome virus
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