In vivo endotoxin exposure increases neutrophil counts without maintenance of primed superoxide anion release in short-term cultures of sheep bone marrow

A short‐term bone marrow culture was established to determine the functional and proliferative responses of neutrophils sampled from sheep exposed to endotoxin in vivo. Iliac crest bone marrow was drawn from surgical control animals and from sheep given a 12‐h infusion of endotoxin. In these bone ma...

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Bibliographic Details
Published in:Journal of leukocyte biology 1994-08, Vol.56 (2), p.145-150
Main Authors: Carey, Lisa A., Albertine, Kurt H., Gee, Marlys H.
Format: Article
Language:English
Subjects:
Analysis of the immune response. Humoral and cellular immunity
Animals
Biological and medical sciences
bone marrow
Bone Marrow - drug effects
Bone Marrow - metabolism
Bone Marrow Cells
Cell interactions
Cell Survival
Cells, Cultured
endotoxin
Endotoxins - pharmacology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Immunobiology
Leukocyte Count - drug effects
Models, Biological
neutrophil
Neutrophils - cytology
Neutrophils - drug effects
Neutrophils - metabolism
priming
Sheep
Stimulation, Chemical
superoxide anion release
Superoxides - metabolism
Tetradecanoylphorbol Acetate - pharmacology
Time Factors
viability
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Summary:A short‐term bone marrow culture was established to determine the functional and proliferative responses of neutrophils sampled from sheep exposed to endotoxin in vivo. Iliac crest bone marrow was drawn from surgical control animals and from sheep given a 12‐h infusion of endotoxin. In these bone marrow aspirates, neutrophils from endotoxin‐treated sheep showed primed superoxide anion release compared with controls when stimulated with phorbol myristate acetate. Priming was no longer present in neutrophils tested after 2 days in culture. The neutrophil cell count, however, was higher after 2 days in culture than in controls. Increased neutrophil production/viability was reproduced in naive bone marrow cultures by replacing part of the normal serum medium with plasma taken from sheep several hours after endotoxin infusion. These data suggest that a dissociation exists between priming and increased neutrophil production/viability in response to endotoxin. J. Leukoc. Biol. 56: 145–150; 1994.
ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.56.2.145