Axenic culture of marine brown alga Sargassum swartzii (Phaeophyta, Fucales)

A method for obtaining axenic culture of Sargassum swartzii from the cut apical and intercalary portions of the thalli and from the sterile oospores has been described. The method involves the sonication, surface sterilization of the thalli with betadiene (1%) for 3 min followed by a treatment with...

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Bibliographic Details
Published in:Indian journal of marine sciences 1994-09, Vol.23 (3), p.165-167
Main Authors: RAMALAKSHMI DATTA, DATTA, B. K, CHAUHAN, V. D
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General agronomy. Plant production
Marine
Plant aquaculture, algal production (macroalgae)
Plant physiology and development
Sargassum swartzii
Tissue cultures, protoplasts
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Summary:A method for obtaining axenic culture of Sargassum swartzii from the cut apical and intercalary portions of the thalli and from the sterile oospores has been described. The method involves the sonication, surface sterilization of the thalli with betadiene (1%) for 3 min followed by a treatment with antibiotic mixture (330 mg neomycin, 150 mg oxytetracycline and 300 mg chloramphenical in liter of autoclaved seawater) for 12-24 h. The sterile oospores are better source of axenic material for this alga.
ISSN:0379-5136
0975-1033