Ang-(1-7) promotes the migration and invasion of human renal cell carcinoma cells via Mas-mediated AKT signaling pathway

Ang-(1-7) is an active peptide component of renin-angiotensin system and endogenous ligand for Mas receptor. In the current study, we showed that Ang-(1-7) enhanced migratory and invasive abilities of renal cell carcinoma cells 786-O and Caki-1 by wound-healing, transwell migration and transwell inv...

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Published in:Biochemical and biophysical research communications 2015-05, Vol.460 (2), p.333-340
Main Authors: Zheng, Shuai, Yang, Ying, Song, Ran, Yang, Xiaomei, Liu, Hua, Ma, Qian, Yang, Longyan, Meng, Ran, Tao, Tao, Wang, Songlin, He, Junqi
Format: Article
Language:English
Subjects:
AKT signaling
Angiotensin I - pharmacology
Angiotensin-(1-7)
Cell Line, Tumor
Humans
Invasion
Kidney Neoplasms - enzymology
Kidney Neoplasms - metabolism
Kidney Neoplasms - pathology
Mas receptor
Migration
Neoplasm Invasiveness
Neoplasm Metastasis
Peptide Fragments - pharmacology
Proto-Oncogene Proteins - metabolism
Proto-Oncogene Proteins c-akt - metabolism
Receptors, G-Protein-Coupled - metabolism
Renal cell carcinoma
Signal Transduction - drug effects
Wound Healing
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Summary:Ang-(1-7) is an active peptide component of renin-angiotensin system and endogenous ligand for Mas receptor. In the current study, we showed that Ang-(1-7) enhanced migratory and invasive abilities of renal cell carcinoma cells 786-O and Caki-1 by wound-healing, transwell migration and transwell invasion assays. Mas antagonist A779 pretreatment or shRNA-mediated Mas knockdown abolished the stimulatory effect of Ang-(1-7). Furthermore, Ang-(1-7)-stimulated AKT activation was inhibited by either A779 pretreatment or Mas knockdown. Blockage of AKT signaling by AKT inhibitor VIII inhibited Ang-(1-7)-induced migration and invasion in 786-O cells. Taken together, our results provided the first evidence for the pro-metastatic role of Ang-(1-7) in RCC, which may help to better understand the molecular mechanism underlying the progression of this tumor. •Ang-(1-7) promotes migration and invasion in RCC cells 786-O and Caki-1.•Mas receptor mediates Ang-(1-7)-induced promotion of migration and invasion.•Ang-(1-7)/Mas-induced cell migration and invasion is dependent on AKT activation.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2015.03.035