BIX-01294-induced autophagy regulates elongation of primary cilia

Previously, we showed that BIX-01294 treatment strongly activates autophagy. Although, the interplay between autophagy and ciliogenesis has been suggested, the role of autophagy in ciliogenesis is controversial and largely unknown. In this study, we investigated the effects of autophagy induced by B...

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Published in:Biochemical and biophysical research communications 2015-05, Vol.460 (2), p.428-433
Main Authors: Shin, Ji Hyun, Kim, Pan Soo, Kim, Eun Sung, Park, So Jung, Jo, Yoon Kyung, Hwang, Jung Jin, Park, Tae Joo, Chang, Jong Wook, Seo, Jin-Ho, Cho, Dong-Hyung
Format: Article
Language:English
Subjects:
ATG6
Autophagy
Autophagy - drug effects
Azepines - pharmacology
BIX-01294
Cell Line, Transformed
Cilia
Cilia - drug effects
Cilia - physiology
Ciliogenesis
Histone-Lysine N-Methyltransferase - antagonists & inhibitors
Humans
Quinazolines - pharmacology
Retinal Pigment Epithelium - cytology
Retinal Pigment Epithelium - drug effects
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Summary:Previously, we showed that BIX-01294 treatment strongly activates autophagy. Although, the interplay between autophagy and ciliogenesis has been suggested, the role of autophagy in ciliogenesis is controversial and largely unknown. In this study, we investigated the effects of autophagy induced by BIX-01294 on the formation of primary cilia in human retinal pigmented epithelial (RPE) cells. Treatment of RPE cells with BIX-01294 caused strong elongation of the primary cilium and increased the number of ciliated cells, as well as autophagy activation. The elongated cilia in serum starved cultured cells were gradually decreased by re-feeding the cells with normal growth medium. However, the disassembly of cilia was blocked in the BIX-01294-treated cells. In addition, both genetic and chemical inhibition of autophagy suppressed BIX-01294-mediated ciliogenesis in RPE cells. Taken together, these results suggest that autophagy induced by BIX-01294 positively regulates the elongation of primary cilium. •BIX-01294 increases autophagy activation in RPE cells.•BIX-01294 increases elongation of primary cilia in RPE cells.•BIX-01294 suppresses cilia disassembly in RPE cells.•Inhibition of autophagy suppresses BIX-01294-mediated elongation of cilia.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2015.03.050