Protective effect of Jolkinolide B on LPS-induced mouse acute lung injury

• Published in: International immunopharmacology 2015-05, Vol.26 (1), p.119-124
• Main Authors: Yang, Hailing, Li, Yan, Huo, Pengfei, Li, Xiao-ou, Kong, Daliang, Mu, Wei, Fang, Wei, Li, Lingxia, Liu, Ning, Fang, Ling, Li, Hongjun, He, Chengyan
• Format: Article
• Language: English
• Subjects: Acute lung injury; Acute Lung Injury - chemically induced; Acute Lung Injury - pathology; Acute Lung Injury - prevention & control; Animals; Anti-Inflammatory Agents - administration & dosage; Anti-Inflammatory Agents - isolation & purification; Anti-Inflammatory Agents - therapeutic use; Blotting, Western; Capillary Permeability - drug effects; Cytokines - immunology; Disease Models, Animal; Diterpenes - administration & dosage; Diterpenes - isolation & purification; Diterpenes - therapeutic use; Enzyme-Linked Immunosorbent Assay; Euphorbia - chemistry; Jolkinolide B; Lipopolysaccharides - pharmacology; Lung - blood supply; Lung - drug effects; Lung - immunology; Lung - pathology; Male; MAPK; Mice, Inbred C57BL; NF-κB; Organ Size - drug effects; Plant Roots - chemistry
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2015.03.021

Jolkinolide B (JB), an ent-abietane diterpenoid, isolated from the dried root of Euphorbia fischeriana, has been reported to have potent anti-tumor and anti-inflammatory activities. However, the effects of JB on acute lung injury (ALI) and underlying molecular mechanisms have not been investigated. The present study aimed to investigate the effect of JB on lipopolysaccharide (LPS)-induced ALI. Male C57BL/6 mice were pretreated with dexamethasone or JB 1h before intranasal instillation of LPS. The results showed that JB markedly attenuated LPS-induced histological alterations, lung edema, inflammatory cell infiltration, myeloperoxidase (MPO) activity as well as the production of TNF-α, IL-6 and IL-1β. Furthermore, JB also significantly inhibited LPS-induced the degradation of IκBα and phosphorylation of NF-κB p65 and MAPK. Therefore, our study provides the first line of evidence that pretreatment of JB has a protective effect on LPS-induced ALI in mice. The anti-inflammatory mechanism of JB may be attributed to its suppression of NF-κB and MAPK activation. •Jolkinolide B (JB) was a ent-abietane diterpenoid, isolated from the dried root of Euphorbia fischeriana.•JB markedly attenuated LPS-induced histological alterations, lung edema, inflammatory cells infiltration, MPO activity.•JB inhibited TNF-α, IL-1β, IL-6 production in BALF of LPS-induced ALI mice.•JB inhibited NF-κB activation and MAPK phosphorylation in LPS-induced ALI mice.