Apoptosis in human hepatoma HepG2 cells induced by corn peptides and its anti-tumor efficacy in H22 tumor bearing mice

• Published in: Food and chemical toxicology 2013-01, Vol.51, p.297-305
• Main Authors: Li, Jiang-Tao, Zhang, Jiu-Liang, He, Hui, Ma, Zhi-Li, Nie, Zhi-Kui, Wang, Zhen-Zhen, Xu, Xiao-Gen
• Format: Article
• Language: English
• Subjects: Animals; Anti-tumor; Antineoplastic Agents, Phytogenic - pharmacology; Apoptosis; Apoptosis - drug effects; Apoptosis - physiology; bioactive properties; Biological and medical sciences; Caspase 3 - metabolism; cell cycle; cell viability; corn; Corn peptides (CPs); Dose-Response Relationship, Drug; Drug Screening Assays, Antitumor; functional foods; Gastroenterology. Liver. Pancreas. Abdomen; Hep G2 Cells - drug effects; Hepatocellular carcinoma; hepatoma; human cell lines; Humans; Immunomodulatory; interferon-gamma; interleukin-2; Interleukin-2 - metabolism; Liver. Biliary tract. Portal circulation. Exocrine pancreas; Male; Medical sciences; Mice; Mice, Inbred BALB C; peptides; Plant Proteins - pharmacology; S Phase Cell Cycle Checkpoints - drug effects; spleen; Spleen - drug effects; Toxicology; tumor necrosis factor-alpha; Tumor Necrosis Factor-alpha - metabolism; Tumors; Zea mays - chemistry
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2012.09.038

► The anti-tumor efficacy of corn peptides (CPs) was evaluated. ► CPs was found to be an apoptosis inducer in HepG2 cells. ► Apoptosis mechanism induced by CPs lay on the increase in Bax/Bcl-2 ratio and the up-regulation of Cleaved-caspase-3, p53. ► The anti-tumor activity of CPs was associated with enhancement of immunization. This study was aimed at evaluating the anti-tumor mechanism of corn peptides (CPs). In vitro, the results showed that CPs significantly inhibited cell viability in both a dose- and a time-dependent manner. CPs treatment induced S cell-cycle arrest and caused apoptotic death in HepG2 cells. It was observed that CPs caused the increased in Bax/Bcl-2 ratio and triggered the activation of Cleaved-caspase-3, p53 in HepG2 cells. In vivo, the results showed that CPs could not only inhibit the growth of the tumor, but also enhance the spleen index, as well as the level of IL-2, IFN-γ and TNF-α. Moreover, CPs could prolong the survival time in H22-bearing mice. This study demonstrated that CPs was an apoptosis inducer in HepG2 cells, that it could effectively inhibit hepatocellular carcinoma in vivo via enhancement of host immune system function, and that it could be a safe and effective anticancer, bioactive agent or functional food.