Topical application of Gallic acid suppresses the 7,12-DMBA/Croton oil induced two-step skin carcinogenesis by modulating anti-oxidants and MMP-2/MMP-9 in Swiss albino mice

• Published in: Food and chemical toxicology 2014-04, Vol.66, p.44-55
• Main Authors: Subramanian, Vimala, Venkatesan, Balaji, Tumala, Anusha, Vellaichamy, Elangovan
• Format: Article
• Language: English
• Subjects: 9,10-Dimethyl-1,2-benzanthracene - toxicity; Administration, Topical; Animals; Anticancer activity; Antineoplastic agents; Antioxidants - pharmacology; Biological and medical sciences; Carcinogenesis, carcinogens and anticarcinogens; Carcinogens - toxicity; Chemical agents; Chemotherapy; Croton; Croton Oil - toxicity; DMBA/Croton oil; Food toxicology; Gallic acid; Gallic Acid - pharmacology; GST; Male; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase 9 - metabolism; Medical sciences; Mice; MMP; Multiple tumors. Solid tumors. Tumors in childhood (general aspects); Papilloma - chemically induced; Papilloma - prevention & control; Pharmacology. Drug treatments; Skin cancer; Skin Neoplasms - chemically induced; Skin Neoplasms - prevention & control; Toxicology; Tumors
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2014.01.017

•Topical application of Gallic acid (GA), a dietary antioxidant delays the DMBA/Croton oil induced skin carcinogenesis.•Topical application of GA normalized the altered levels of serum LDH-isoenzymes profile, enzymatic and non-anti-oxidants.•GA treatment suppresses the MMPs and collagen production in two-step skin carcinogenesis.•Underlying mechanism of action of GA probably due to its antioxidant & anti-fibrotic activity. Gallic acid (GA – 3,4,5-trihydroxybenzoic acid), a dietary anti-oxidant has been shown to inhibit cancer cell growth in in vitro. Herein, we investigated the in vivo chemo preventive activity of GA on 7,12-Dimethylbenz[a]anthracene (DMBA)/Croton oil induced two-step skin carcinogenesis in Swiss albino mice. Skin tumor incidence and tumor volume were recorded during the 16weeks of experimental period. In addition, LDH-isozyme shift, skin collagen content, activities of matrix metalloproteinases (MMP-2/MMP-9) enzymes and enzymatic and non-enzymatic antioxidant were studied in the skin and serum of experimental mice. Tumor incidence was significantly increased in the DMBA/Croton oil induced mice (100%; p