Novel co-enrichment method for isolation of magnetotactic bacteria

A novel co‐enrichment technique was designed for enrichment of magnetotactic bacteria from soil, water, and sediments. Delayed addition of iron uptake inducer and the iron source proved amenable to induce magnetosome synthesis by MTB followed by their separation from consortium using magnetic flux....

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Bibliographic Details
Published in:Journal of basic microbiology 2015-04, Vol.55 (4), p.520-526
Main Authors: Sorty, Ajay M., Shaikh, Nasir R.
Format: Article
Language:English
Subjects:
Bacteria
Bacteriological Techniques
DNA, Bacterial
Geologic Sediments - microbiology
Gram-Negative Bacteria - growth & development
Gram-Negative Bacteria - isolation & purification
Gram-Positive Bacteria - growth & development
Gram-Positive Bacteria - isolation & purification
India
Isolation technique
Magnetic Fields
Magnetosomes - metabolism
Magnetotactic bacteria
Magnetotaxis
Microbial Consortia
Phylogeny
Sediment
Soil
Soil Microbiology
Stress, Physiological
Water Microbiology
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Summary:A novel co‐enrichment technique was designed for enrichment of magnetotactic bacteria from soil, water, and sediments. Delayed addition of iron uptake inducer and the iron source proved amenable to induce magnetosome synthesis by MTB followed by their separation from consortium using magnetic flux. We successfully enriched and isolated both North seeking as well as South seeking magnetotactic bacteria from Lonar Lake (Buldhana), Moti Lake (Jalna), Ghanewadi Lake (Jalna), Ganesh Lake (Miraj), Rankala Lake (Kolhapur), and industrial metal‐contaminated glaying soils (Jalna) and a soil (Karad), (MS, India) exposed to high‐voltage electric current. The hanging drop preparations and growth under magnetic stress on low‐agar media allowed conformation of magnetotactic behavior of the isolates. Both Gram positive and Gram negative MTB were isolated with diverse morphologies. South seeking population was more predominant. The soil inhabitants showed little dwelling property which was more prominent in case of aquatic inhabitants. The use of in situ pH and salt concentrations during enrichment and isolation found suited. The simultaneous growth of whole consortium in the system ensured the in situ simulation of microenvironment needful for proper growth of fastidious MTB.
ISSN:0233-111X
1521-4028
DOI:10.1002/jobm.201400457