Novel co-enrichment method for isolation of magnetotactic bacteria

• Published in: Journal of basic microbiology 2015-04, Vol.55 (4), p.520-526
• Main Authors: Sorty, Ajay M., Shaikh, Nasir R.
• Format: Article
• Language: English
• Subjects: Bacteria; Bacteriological Techniques; DNA, Bacterial; Geologic Sediments - microbiology; Gram-Negative Bacteria - growth & development; Gram-Negative Bacteria - isolation & purification; Gram-Positive Bacteria - growth & development; Gram-Positive Bacteria - isolation & purification; India; Isolation technique; Magnetic Fields; Magnetosomes - metabolism; Magnetotactic bacteria; Magnetotaxis; Microbial Consortia; Phylogeny; Sediment; Soil; Soil Microbiology; Stress, Physiological; Water Microbiology
• ISSN: 0233-111X; 1521-4028
• DOI: 10.1002/jobm.201400457

A novel co‐enrichment technique was designed for enrichment of magnetotactic bacteria from soil, water, and sediments. Delayed addition of iron uptake inducer and the iron source proved amenable to induce magnetosome synthesis by MTB followed by their separation from consortium using magnetic flux. We successfully enriched and isolated both North seeking as well as South seeking magnetotactic bacteria from Lonar Lake (Buldhana), Moti Lake (Jalna), Ghanewadi Lake (Jalna), Ganesh Lake (Miraj), Rankala Lake (Kolhapur), and industrial metal‐contaminated glaying soils (Jalna) and a soil (Karad), (MS, India) exposed to high‐voltage electric current. The hanging drop preparations and growth under magnetic stress on low‐agar media allowed conformation of magnetotactic behavior of the isolates. Both Gram positive and Gram negative MTB were isolated with diverse morphologies. South seeking population was more predominant. The soil inhabitants showed little dwelling property which was more prominent in case of aquatic inhabitants. The use of in situ pH and salt concentrations during enrichment and isolation found suited. The simultaneous growth of whole consortium in the system ensured the in situ simulation of microenvironment needful for proper growth of fastidious MTB.