molecular signature and spermatogenesis potential of newborn chicken spermatogonial stem cells in vitro

Although chicken spermatogonial stem cells (SSCs) have received considerable attention in recent years, only a few studies so far have focused on their derivation and characterization in vitro. Identification of specific molecular biomarkers and differentiation capacity of chicken SSCs would not onl...

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Published in:In vitro cellular & developmental biology. Animal 2015-04, Vol.51 (4), p.415-425
Main Authors: Sisakhtnezhad, Sajjad, Bahrami, Ahmad Reza, Matin, Maryam M, Dehghani, Hesam, Momeni-Moghaddam, Madjid, Boozarpour, Sohrab, Farshchian, Moein, Dastpak, Mahtab
Format: Article
Language:English
Subjects:
alkaline phosphatase
Alkaline Phosphatase - metabolism
Animal Genetics and Genomics
Animals
Animals, Newborn
Biomarkers
Biomedical and Life Sciences
Cell Biology
Cell Culture
Cell Differentiation
Cells, Cultured
Chickens
Developmental Biology
gene expression
Gene Expression Regulation
Lewis X Antigen - genetics
Life Sciences
Male
messenger RNA
molecular biology
neonates
proteins
RNA, Messenger - analysis
Spermatogenesis
Spermatogonia - cytology
STEM CELLS
Stem Cells - cytology
Stem Cells - physiology
Testis - cytology
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Summary:Although chicken spermatogonial stem cells (SSCs) have received considerable attention in recent years, only a few studies so far have focused on their derivation and characterization in vitro. Identification of specific molecular biomarkers and differentiation capacity of chicken SSCs would not only help us to understand cell and molecular biology of these cells, but also can contribute to their applications in biotechnology. In this regard, we found that colony-forming cells (SSCs) in newborn chicken testicular cell cultures were positive for alkaline phosphatase activity and also expressed specific markers including DAZL, STRA-8, CVH, PLZF, SPRY-1, GFRα1, GDNF, POU5F1, NANOG, GPR125, THY-1, c-KIT, and BCL6B, at mRNA level. Moreover, these cells expressed POU5F1 and GPR125 proteins as reliable intracellular and cell surface markers, respectively; whereas they were negative for SSEA-1. Furthermore, we showed that newborn chicken colony-forming cells had spermatogenesis potential and thus could be produced sperm-like cells in a three-dimensional matrix in vitro. In conclusion, this study reports novel insights into the molecular signature of newborn chicken SSCs in comparison with mammalian SSCs and for the first time we report a successful protocol for in vitro spermatogenesis and thus production of sperm-like cells from newborn chicken testicular cell cultures.
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-014-9843-1