Temperate bacteriophages collected by outer membrane vesicles in Komagataeibacter intermedius

The acetic acid bacteria have mainly relevance for bacterial cellulose production and fermented bio‐products manufacture. The purpose of this study was to identify temperate bacteriophages in a cellulose‐producing bacterial strain Komagataeibacter intermedius IMBG180. Prophages from K. intermedius I...

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Bibliographic Details
Published in:Journal of basic microbiology 2015-04, Vol.55 (4), p.509-513
Main Authors: Kharina, Alla, Podolich, Olga, Faidiuk, Iuliia, Zaika, Sergiy, Haidak, Andriy, Kukharenko, Olga, Zaets, Iryna, Tovkach, Fedor, Reva, Oleg, Kremenskoy, Maxim, Kozyrovska, Natalia
Format: Article
Language:English
Subjects:
Acetobacteraceae - isolation & purification
Acetobacteraceae - physiology
Acetobacteraceae - ultrastructure
Acetobacteraceae - virology
Bacteria
Bacteriophages
DNA, Viral - analysis
Microscopy, Electron, Transmission
Mitomycin - pharmacology
Myoviridae
Myoviridae - classification
Myoviridae - isolation & purification
Myoviridae - physiology
Myoviridae - ultrastructure
Nalidixic Acid - pharmacology
Outer membrane vesicles
Phylogeny
Prophages - physiology
Prophages - ultrastructure
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Summary:The acetic acid bacteria have mainly relevance for bacterial cellulose production and fermented bio‐products manufacture. The purpose of this study was to identify temperate bacteriophages in a cellulose‐producing bacterial strain Komagataeibacter intermedius IMBG180. Prophages from K. intermedius IMBG180 were induced with mitomycin C and nalidixic acid. Transmission electron microscopy analysis exhibited tailed bacteriophages belonging to Myoviridae. A PCR assay targeting the capsid gene of the myoviruses proved phylogenetic position of induced phages. Nalidixic acid was poor inducer of prophages, however, it induced the OMV‐like particles release. Size of OMVs depended on an antibiotic applied for phage induction and varied in the range of 30–80 and 120–200 nm. Inside some of them, tails of phages have been visible. Under conditions, inducing prophages, OMVs acted as the collectors of formed phage particles, using outer membrane receptors for phage detection (in this case, outer membrane siderophore receptor), and fulfilled therefore “a cleaning,” as well as defensive functions, preventing bacteriophage spread outside population. This is the first description of myoviruses affiliated to K. intermedius, as well as outer membrane vesicles interaction with phages within this host.
ISSN:0233-111X
1521-4028
DOI:10.1002/jobm.201400711