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Expression of the myostatin gene in the adductor muscle of the Pacific lion-paw scallop Nodipecten subnodosus in association with growth and environmental conditions
ABSTRACT The cDNA sequence of the myostatin gene in the Pacific lion‐paw Nodipecten subnodosus (Ns‐mstn) was characterized, and the temporal expression during grow‐out was analyzed for the first time in a scallop. Ns‐mstn encodes a 459‐amino‐acid protein in which two propeptide proteolytic sites wer...
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Published in: | Journal of experimental zoology. Part A, Ecological and integrative physiology Ecological and integrative physiology, 2015-04, Vol.323 (4), p.239-255 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | ABSTRACT
The cDNA sequence of the myostatin gene in the Pacific lion‐paw Nodipecten subnodosus (Ns‐mstn) was characterized, and the temporal expression during grow‐out was analyzed for the first time in a scallop. Ns‐mstn encodes a 459‐amino‐acid protein in which two propeptide proteolytic sites were identified, the previously recognized (RSKR) and a second one at position 266–269 aa (RRKR). The alternative furin cleavage site could be related with post‐translational processing, or it could be a tissue‐specific mechanism for signaling activity. The Ns‐mstn transcript was located by in situ hybridization in sarcomeres and around the nucleus of muscle fibers. The temporal expression analysis by qPCR in the adductor muscle showed that Ns‐mstn expression was significantly different (P |
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ISSN: | 1932-5223 2471-5638 1932-5231 2471-5646 |
DOI: | 10.1002/jez.1914 |