Isolation, characterization and mapping of pyrimidine auxotrophs of Phycomyces blakesleeanus

A total of seven pyrimidine auxotrophs of Phycomyces were isolated from among 5-fluoroorotate acid (5-FOA)-resistant mutants. They were classifed by complementation into two groups. A representative mutant strain belonging to one group was deficient in orotate phosphoribosyl transferase (OPRTase; EC...

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Bibliographic Details
Published in:Current genetics 1993-12, Vol.24 (6), p.515-519
Main Authors: CAMPUZANO, V, DEL VALLE, P, DE VICENTE, J. I, ESLAVA, A. P, ALVAREZ, M. I
Format: Article
Language:English
Subjects:
analisis microbiologico
analyse microbiologique
auxotrofia
auxotrophie
auxotrophy
Biological and medical sciences
carte genetique
Cell-Free System
Classical genetics, quantitative genetics, hybrids
Drug Resistance, Microbial
Fundamental and applied biological sciences. Psychology
Genetic Complementation Test
Genetic Linkage
genetic maps
Genetic Markers
Genetics of eukaryotes. Biological and molecular evolution
Genotype
mapas geneticos
microbiological analysis
Orotate Phosphoribosyltransferase - genetics
Orotic Acid - analogs & derivatives
Orotic Acid - toxicity
phycomyces
Phycomyces - genetics
Phycomyces - isolation & purification
Phycomyces - metabolism
Phycomyces blakesleeanus
pirimidinas
pyrimidine
pyrimidines
Pyrimidines - metabolism
recombinacion
recombinaison
recombination
Recombination, Genetic
Restriction Mapping
Spores, Fungal - metabolism
Thallophyta, bryophyta
Vegetals
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Summary:A total of seven pyrimidine auxotrophs of Phycomyces were isolated from among 5-fluoroorotate acid (5-FOA)-resistant mutants. They were classifed by complementation into two groups. A representative mutant strain belonging to one group was deficient in orotate phosphoribosyl transferase (OPRTase; EC 2.4.2.10) activity; the mutant strain belonging to the second group was deficient in orotidine-5'-monophosphate decarboxylase (OMPdecase; EC 4.1.1.23). These mutants are defective in the genes pyrF and pyrG respectively. The results from random spore analysis, tetrad analysis, and genecentromere distances showed that these two markers are located in linkage group 6, with pyrG being a proximal marker and pyrF a distal one.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00351715