Multilocus enzyme electrophoretic analysis and DNA‐DNA reassociation of strains designated Prevotella intermedia

Strains of Prevotella intermedia which have been characterized previously by a variety of biochemical and chemical techniques were subjected to multilocus enzyme electrophoresis and DNA‐DNA reassociation. Two separate groups of strains were discernible. One had high homology with the type strain ATC...

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Bibliographic Details
Published in:Letters in applied microbiology 1992-07, Vol.15 (1), p.8-11
Main Authors: Shah, H.N., Gharbia, S.E.
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Microbiology
Prevotella intermedia
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Summary:Strains of Prevotella intermedia which have been characterized previously by a variety of biochemical and chemical techniques were subjected to multilocus enzyme electrophoresis and DNA‐DNA reassociation. Two separate groups of strains were discernible. One had high homology with the type strain ATCC 25611 DNA probe and electrophoretically fast migrating malate dehydrogenase (MDH) (3.8–4.0 cm) and glutamate dehydrogenase (GDH) (3.2–3.4 cm) bands. The other group of strains hybridized with the DNA probe of reference strain ATCC 33563 and possessed slower moving enzymes (MDH, 3.0 cm; GDH, 1.4–1.6 cm). These results indicate that strains currently identified as P. intermedia comprise at least two geno‐species, and that the criteria used to define this species are inadequate.
ISSN:0266-8254
1472-765X
DOI:10.1111/j.1472-765X.1992.tb00710.x