Proteoglycans from the vertebral cartilage of the clearnose skate, Raja eglanteria: Inhibition of hydroxyapatite formation

Studies were undertaken to examine the importance of organic proteoglycan matrices in the calcification of elasmobranch vertebral cartilage. Proteoglycans were extracted from the vertebral cartilage of the clearnose skate, Raja eglanteria Bosc, with a 3M guanidine hydrochloride/10% EDTA solution. Pr...

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Bibliographic Details
Published in:Fish physiology and biochemistry 1995-06, Vol.14 (3), p.247-251
Main Authors: Gelsleichter, J J, Musick, J A, Van Veld, P
Format: Article
Language:English
Subjects:
Marine
Raja eglanteria
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Summary:Studies were undertaken to examine the importance of organic proteoglycan matrices in the calcification of elasmobranch vertebral cartilage. Proteoglycans were extracted from the vertebral cartilage of the clearnose skate, Raja eglanteria Bosc, with a 3M guanidine hydrochloride/10% EDTA solution. Proteoglycan solutions (12 μg ml(-1)) were effective inhibitors of hydroxyapatite formation in vitro from high concentration calcium phosphate solutions. Inhibition of crystal formation appears to occur through the restriction of phase transformation from a calcium phosphate precursor to hydroxyapatite crystals. The concentration and/or degradation of proteoglycans in elasmobranch vertebral cartilage may have a physiological role in the production of alternating mineral-rich and mineral-poor growth zones, currently used in ageing studies.
ISSN:0920-1742
1573-5168
DOI:10.1007/BF00004315