A linkage map of diploid Avena based on RFLP loci and a locus conferring resistance to nine isolates of Puccinia coronata var. 'avenae'

• Published in: Theoretical and applied genetics 1994-12, Vol.89 (7-8), p.831-837
• Main Authors: Rayapati, P.J, Gregory, J.W, Lee, M, Wise, R.P. (Iowa State Univ., Ames (USA). Dept. of Agronomy)
• Format: Article
• Language: English
• Subjects: ADN; AVENA; Avena strigosa; Avena wiestii; Biological and medical sciences; CARTE GENETIQUE; Classical genetics, quantitative genetics, hybrids; Disease resistance; ENZIMA DE RESTRICCION; ENZYME DE RESTRICTION; Fundamental and applied biological sciences. Psychology; GENE; GENES; Genetics of eukaryotes. Biological and molecular evolution; linkage map; LOCI; LOCUS; MAPAS GENETICOS; Monocots; Pflanzenzuechtung; POLIMORFISMO GENETICO; POLYMORPHISME GENETIQUE; Pteridophyta, spermatophyta; PUCCINIA CORONATA; RESISTANCE AUX MALADIES; RESISTENCIA A LA ENFERMEDAD; RFLP; SECUENCIA NUCLEICA; SEQUENCE NUCLEIQUE; Vegetals
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/BF00224505

An F2 oat population was produced by crossing the diploid (n=7) species Avena strigosa (CI 3815) with Avena wiestii (CI 1994), resistant and susceptible, respectively, to 40 isolates of Puccinia coronata, the causal agent of crown rust. 88 F2 individuals were used to construct an RFLP linkage map representing the A genome of cultivated hexaploid oat. 208 RFLP loci have been placed into 10 linkage groups. This map covers 2416 cM, with an average of 12 cM between RFLP loci. 88 F3 lines, derived from F2 individuals used to construct the map, were screened for resistance to 9 isolates of P. coronata. One locus, Pca, was found to confer a dominant resistance phenotype to isolates 203, 258, 263, 264B, 290, 298, 325A, and 345. Pca also conferred resistance to isolate 276; however, an unlinked second gene may also be involved.