Positive impact of sucrose supplementation during slow freezing of cat ovarian tissues on cellular viability, follicle morphology, and DNA integrity

The objectives of the study were to (1) examine and optimize the impact of sucrose during slow freezing and (2) compare the results of two freezing methods (slow freezing and vitrification) on cellular viability (germinal and stromal cells), follicle morphology, DNA integrity, and gap junction prote...

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Bibliographic Details
Published in:Theriogenology 2015-06, Vol.83 (9), p.1553-1561
Main Authors: Tanpradit, Nae, Comizzoli, Pierre, Srisuwatanasagul, Sayamon, Chatdarong, Kaywalee
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
Cats
Cell Survival
Connexin 43
Cryopreservation
Cryopreservation - methods
Cryopreservation - veterinary
DNA Damage
Domestic cat
Female
Ovarian Follicle - anatomy & histology
Ovarian Follicle - drug effects
Ovarian Follicle - physiology
Ovarian tissue
Ovary - physiology
Sucrose
Sucrose - pharmacology
Vitrification
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Summary:The objectives of the study were to (1) examine and optimize the impact of sucrose during slow freezing and (2) compare the results of two freezing methods (slow freezing and vitrification) on cellular viability (germinal and stromal cells), follicle morphology, DNA integrity, and gap junction protein expression (connexin 43 [Cx 43]). Different sucrose supplementations (0, 0.1, and 0.3 M) in standard freezing medium were compared before and after slow freezing. Ovarian tissue slow frozen using 0.1- (4.0 ± 0.4) or 0.3-M sucrose (3.9 ± 0.5) yielded better follicular viability (number of positive follicles per 0.0625 mm2) than the group without sucrose (1.9 ± 0.2; P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2015.01.035