The fate of recombinant chromosomes and genome interaction in Nicotiana asymmetric somatic hybrids and their sexual progeny

Genomic in-situ hybridization (GISH) was used to monitor the behaviour of parental genomes, and the fate of intergenomic chromosome translocations, through meiosis of plants regenerated from asymmetric somatic hybrids between Nicotiana sylvestris and Nicotiana plumbaginifolia. Meiotic pairing in the...

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Bibliographic Details
Published in:Theoretical and applied genetics 1994-10, Vol.89 (4), p.488-497
Main Authors: Parokonny, A.S. (Royal Botanic Gardens, Surrey (United Kingdom). Jodrell Lab.), Kenton, A, Gleba, Y.Y, Bennett, M.D
Format: Article
Language:English
Subjects:
DESCENDANCE
GENOMAS
GENOME
HIBRIDACION INTERESPECIFICA
HIBRIDO SOMATICO
HYBRIDATION INTERSPECIFIQUE
HYBRIDE SOMATIQUE
MEIOSE
MEIOSIS
NICOTIANA
Nicotiana plumbaginifolia
Nicotiana sylvestris
PROGENIE
RECOMBINACION
RECOMBINAISON
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Summary:Genomic in-situ hybridization (GISH) was used to monitor the behaviour of parental genomes, and the fate of intergenomic chromosome translocations, through meiosis of plants regenerated from asymmetric somatic hybrids between Nicotiana sylvestris and Nicotiana plumbaginifolia. Meiotic pairing in the regenerants was exclusively between chromosomes or chromosome segments derived from the same species. Translocation (recombinant) chromosomes contained chromosome segments from both parental species, and were detected at all stages of meiosis. They occasionally paired with respectively homologous segments of N. sylvestris or N. plumbaginifolia chromosomes. Within hybrid nuclei, the meiotic division of N. plumbaginifolia lagged behind that of N. sylvestris. However, normal and recombinant chromosomes were eventually incorporated into dyads and tetrads, and the regenerants were partially pollen fertile. Recombinant chromosomes were transmitted through either male or female gametes, and were detected by GISH in sexual progeny obtained on selfing or backcrossing the regenerants to N. sylvestris. A new recombinant chromosome in one plant of the first backcross generation provided evidence of further chromosome rearrangements occurring at, or following, meiosis in the original regenerant. This study demonstrates the stable incorporation of chromosome segments from one parental genome of an asymmetric somatic hybrid into another, via intergenomic translocation, and reveals their transmission to subsequent sexual progeny.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00225385