Identification and characterization of a novel tyrosine kinase from megakaryocytes

Protein-tyrosine kinases play pivotal roles in cell signal transduction. We have isolated a cDNA clone encoding a novel human intracytoplasmic tyrosine kinase, termed matk (megakaryocyte-associated tyrosine kinase). Expression of matk mRNA was predominantly found in cells of megakaryocytic lineage....

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Bibliographic Details
Published in:The Journal of biological chemistry 1994-01, Vol.269 (2), p.1068-1074
Main Authors: Bennett, B D, Cowley, S, Jiang, S, London, R, Deng, B, Grabarek, J, Groopman, J E, Goeddel, D V, Avraham, H
Format: Article
Language:English
Subjects:
amino acid sequence
Analytical, structural and metabolic biochemistry
Base Sequence
Biological and medical sciences
cDNA
Cloning, Molecular
Cytoplasm - enzymology
DNA Primers - chemistry
DNA, Complementary - genetics
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Gene Expression
genes
Humans
man
matk gene
megakaryocytes
Megakaryocytes - enzymology
Molecular Sequence Data
nucleotide sequence
platelets
predictions
protein-tyrosine kinase
Protein-Tyrosine Kinases - genetics
Proto-Oncogene Proteins pp60(c-src)
RNA, Messenger - genetics
Sequence Alignment
Sequence Homology, Amino Acid
Tissue Distribution
Transferases
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Summary:Protein-tyrosine kinases play pivotal roles in cell signal transduction. We have isolated a cDNA clone encoding a novel human intracytoplasmic tyrosine kinase, termed matk (megakaryocyte-associated tyrosine kinase). Expression of matk mRNA was predominantly found in cells of megakaryocytic lineage. The matk cDNA clone encodes a polypeptide of 527 amino acids and has closest sequence similarity to the csk tyrosine kinase. Sequence comparisons also indicate that matk contains src homology region 2 and 3 domains but lacks the NH2-terminal myristylation signal, the negative regulatory tyrosine (Tyr-527), and the autophosphorylation site (Tyr-416) corresponding to those found in src. Antibodies raised against the NH2 terminus of matk immunoprecipitated a 60-kDa protein from the CMK human megakaryocyte cell line. Expression of matk mRNA was up-regulated in megakaryocytic cells induced to differentiate by the phorbol ester. Based on its restriction in expression and its modulation during in vitro differentiation, it is likely that matk participates in signal transduction during megakaryocytopoiesis.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(17)42222-8