Bioconjugated persistent luminescence nanoparticles for Föster resonance energy transfer immunoassay of prostate specific antigen in serum and cell extracts without in situ excitation

A novel Föster resonance energy transfer (FRET) immunoassay based on persistent luminescence nanoparticles (PLNP) was established for prostate specific antigen (PSA) detection in serum and cell extracts without in situ excitation. The specific FRET behavior allows highly selective and sensitive rati...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2015-03, Vol.51 (18), p.3903-3906
Main Authors: Wu, Bo-Yue, Yan, Xiu-Ping
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - chemistry
Antigens
Cell Extracts
Cell Line, Tumor
Energy transfer
Fluorescence Resonance Energy Transfer
Fluorescent Dyes - chemistry
Fretting
Humans
Immunoassay
Luminescence
Microscopy, Electron, Transmission
Nanoparticles
Nanoparticles - chemistry
Nanoparticles - ultrastructure
Prostate
Prostate-Specific Antigen - blood
Prostate-Specific Antigen - immunology
Prostate-Specific Antigen - metabolism
Rhodamines - chemistry
Serums
Spectroscopy, Fourier Transform Infrared
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Summary:A novel Föster resonance energy transfer (FRET) immunoassay based on persistent luminescence nanoparticles (PLNP) was established for prostate specific antigen (PSA) detection in serum and cell extracts without in situ excitation. The specific FRET behavior allows highly selective and sensitive ratiometric photoluminescent detection of PSA in biological samples.
ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc00286a