Production methods for viral particles

Viral particles and virus-like particles (VLPs) or capsids are becoming important vehicles and templates in bio-imaging, drug delivery and materials sciences. Viral particles are prepared by infecting the host organism but VLPs are obtained from cells that express a capsid protein. Some VLPs are dis...

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Bibliographic Details
Published in:Biotechnology letters 2015-04, Vol.37 (4), p.753-760
Main Authors: Machida, Kodai, Imataka, Hiroaki
Format: Article
Language:English
Subjects:
Applied Microbiology
Assembly
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cell-Free System
Chemical engineering
Dismantling
Drug delivery systems
Genetic engineering
Genetics
Life Sciences
Microbiology
Organisms
Production methods
Protein synthesis
Review
Vehicles
Virion - growth & development
Virion - isolation & purification
Virosomes - isolation & purification
Virosomes - metabolism
Virus Cultivation - methods
Viruses
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Summary:Viral particles and virus-like particles (VLPs) or capsids are becoming important vehicles and templates in bio-imaging, drug delivery and materials sciences. Viral particles are prepared by infecting the host organism but VLPs are obtained from cells that express a capsid protein. Some VLPs are disassembled and then re-assembled to incorporate a material of interest. Cell-free systems, which are amenable to manipulating the viral assembly process, are also available for producing viral particles. Regardless of the production system employed, the particles are functionalized by genetic and/or chemical engineering. Here, we review various methods for producing and functionalizing viral particles and VLPs, and we discuss the merits of each system.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-014-1741-9