Morphological and chemical changes in dentin after using endodontic agents: fourier transform Raman spectroscopy, energy-dispersive x-ray fluorescence spectrometry, and scanning electron microscopy study

We examine the morphological and chemical changes in the pulp chamber dentin after using endodontic agents by scanning electron microscopy (SEM), Fourier transform Raman spectroscopy (FT-Raman), and micro energy-dispersive x-ray fluorescence spectrometry (μEDXRF). Thirty teeth were sectioned exposin...

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Bibliographic Details
Published in:Journal of biomedical optics 2012-07, Vol.17 (7), p.075008-1-075008-2
Main Authors: Pascon, Fernanda Miori, Kantovitz, Kamila Rosamilia, Soares, Luís Eduardo Silva, Santo, Ana Maria do Espírito, Martin, Airton Abrahão, Puppin-Rontani, Regina Maria
Format: Article
Language:English
Subjects:
Calcium
Chambers
Dentin
Dentin - chemistry
Dentin - drug effects
Dentin - ultrastructure
Fourier transforms
Humans
Materials Testing
Microscopy, Electron, Scanning
Molecular Conformation - radiation effects
Mouthwashes - pharmacology
Root Canal Irrigants - pharmacology
Scanning electron microscopy
Spectrometry
Spectrometry, X-Ray Emission
Spectroscopy
Spectroscopy, Fourier Transform Infrared
X-ray fluorescence
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Summary:We examine the morphological and chemical changes in the pulp chamber dentin after using endodontic agents by scanning electron microscopy (SEM), Fourier transform Raman spectroscopy (FT-Raman), and micro energy-dispersive x-ray fluorescence spectrometry (μEDXRF). Thirty teeth were sectioned exposing the pulp chamber and divided by six groups (n=5): NT-no treatment; CHX-2% chlorhexidine; CHXE-2% chlorhexidine+17% EDTA; E-17% EDTA; SH5-5.25% NaOCl; SH5E-5.25% NaOCl+17% EDTA. The inorganic and organic content was analyzed by FT-Raman. μEDXRF examined calcium (Ca) and phosphorus (P) content as well as Ca/P ratio. Impressions of specimens were evaluated by SEM. Data were submitted to Kruskal-Wallis and Dunn tests (pNT=SH5E>CHX>E>CHXE). CHXE and E presented the highest Ca/P ratio values compared to the other groups (p
ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.17.7.075008