Synthesis, characterization and in vitro evaluation of cytotoxicity and antimicrobial activity of chitosan-metal nanocomposites

BACKGROUND The present study explores the synthesis of chitosan–metal nanocomposites in view of their increasing application as antimicrobial material. RESULTS Chitosan nanoparticles were prepared by ionic gelation between chitosan and sodium tripolyphosphate. Copper sulphate hydrate (CuSO4.5H2O) an...

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Bibliographic Details
Published in:Journal of chemical technology and biotechnology (1986) 2015-05, Vol.90 (5), p.867-873
Main Authors: Kaur, Pawan, Thakur, Rajesh, Barnela, Manju, Chopra, Meenu, Manuja, Anju, Chaudhury, Ashok
Format: Article
Language:English
Subjects:
Alternaria
Antiinfectives and antibacterials
antimicrobial
Aspergillus flavus
Biotechnology
chitosan
cytotoxicity
Differential scanning calorimetry
In vitro testing
Inhibition
Micrococcus luteus
Nanocomposites
percentage inhibition
Pseudomonas aeruginosa
Rhizoctonia solani
Salmonella enterica
Scanning electron microscopy
Synthesis
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Summary:BACKGROUND The present study explores the synthesis of chitosan–metal nanocomposites in view of their increasing application as antimicrobial material. RESULTS Chitosan nanoparticles were prepared by ionic gelation between chitosan and sodium tripolyphosphate. Copper sulphate hydrate (CuSO4.5H2O) and zinc acetate (Zn (O2CCH3)2) were used as precursors for synthesis of chitosan–copper nanocomposites (Cu/Ch) and chitosan–zinc nanocomposites (Zn/Ch), respectively. Synthesis of nanocomposites was confirmed by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy with energy dispersive X‐ray microanalyses (SEM‐EDX) and differential scanning calorimetry (DSC). Cytotoxicity of nanoformulations was studied by Resazurin assay on Vero cell line (African green monkey kidney cell line). Their antibacterial activities were assessed by the zone of inhibition method and time dependent growth curve against Micrococcus luteus MTCC 1809, Pseudomonas aeruginosa MTCC 424 and Salmonella enterica MTCC 1253 in vitro. Antifungal activity was also studied against Alternaria alterneta, Rhizoctonia solani and Aspergillus flavus in vitro by the mycelium inhibition method. CONCLUSIONS It was observed that all nanoformulations show high antimicrobial activity against all test microorganisms. So chitosan–metal complexes could be promising candidates for novel antimicrobial agents in cosmetic, food and textile industries. © 2014 Society of Chemical Industry
ISSN:0268-2575
1097-4660
DOI:10.1002/jctb.4383