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Synthesis, characterization and in vitro evaluation of cytotoxicity and antimicrobial activity of chitosan-metal nanocomposites
BACKGROUND The present study explores the synthesis of chitosan–metal nanocomposites in view of their increasing application as antimicrobial material. RESULTS Chitosan nanoparticles were prepared by ionic gelation between chitosan and sodium tripolyphosphate. Copper sulphate hydrate (CuSO4.5H2O) an...
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| Published in: | Journal of chemical technology and biotechnology (1986) 2015-05, Vol.90 (5), p.867-873 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c5083-e6e3f5427b5bf724d6bd8f1b9f094fd3973175004147f13b4630f25ac828c1103 |
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| cites | cdi_FETCH-LOGICAL-c5083-e6e3f5427b5bf724d6bd8f1b9f094fd3973175004147f13b4630f25ac828c1103 |
| container_end_page | 873 |
| container_issue | 5 |
| container_start_page | 867 |
| container_title | Journal of chemical technology and biotechnology (1986) |
| container_volume | 90 |
| creator | Kaur, Pawan Thakur, Rajesh Barnela, Manju Chopra, Meenu Manuja, Anju Chaudhury, Ashok |
| description | BACKGROUND
The present study explores the synthesis of chitosan–metal nanocomposites in view of their increasing application as antimicrobial material.
RESULTS
Chitosan nanoparticles were prepared by ionic gelation between chitosan and sodium tripolyphosphate. Copper sulphate hydrate (CuSO4.5H2O) and zinc acetate (Zn (O2CCH3)2) were used as precursors for synthesis of chitosan–copper nanocomposites (Cu/Ch) and chitosan–zinc nanocomposites (Zn/Ch), respectively. Synthesis of nanocomposites was confirmed by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy with energy dispersive X‐ray microanalyses (SEM‐EDX) and differential scanning calorimetry (DSC). Cytotoxicity of nanoformulations was studied by Resazurin assay on Vero cell line (African green monkey kidney cell line). Their antibacterial activities were assessed by the zone of inhibition method and time dependent growth curve against Micrococcus luteus MTCC 1809, Pseudomonas aeruginosa MTCC 424 and Salmonella enterica MTCC 1253 in vitro. Antifungal activity was also studied against Alternaria alterneta, Rhizoctonia solani and Aspergillus flavus in vitro by the mycelium inhibition method.
CONCLUSIONS
It was observed that all nanoformulations show high antimicrobial activity against all test microorganisms. So chitosan–metal complexes could be promising candidates for novel antimicrobial agents in cosmetic, food and textile industries. © 2014 Society of Chemical Industry |
| doi_str_mv | 10.1002/jctb.4383 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1677907780</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1677907780</sourcerecordid><originalsourceid>FETCH-LOGICAL-c5083-e6e3f5427b5bf724d6bd8f1b9f094fd3973175004147f13b4630f25ac828c1103</originalsourceid><addsrcrecordid>eNqN0c-L1DAUB_AgCo6rB_-DgheF7e5Lkybp0R3c8ceqiCN6C2kmYTK2yZhkxq0X_3Xb7bIHQfAUyPt8H3l5CD3FcIYBqvOdzu0ZJYLcQwsMDS8pY3AfLaBioqxqXj9Ej1LaAQATFVug358Hn7cmuXRa6K2KSmcT3S-VXfCF8pvC-eLocgyFOaruMN8HW-ghhxyunXZ5uHHKZ9c7HUPrVFeMbdxxKk1063JIype9yWPJKx906PchuWzSY_TAqi6ZJ7fnCfpy-Wq9fF1efVy9Wb68KnUNgpSGGWJrWvG2bi2v6Ia1G2Fx21hoqN2QhhPMawCKKbeYtJQRsFWttKiExhjICXo-993H8ONgUpa9S9p0nfImHJLEjPMGOBf_RQkRVFA80md_0V04RD8OMirGBReMT-rFrMbfSSkaK_fR9SoOEoOctianrclpa6M9n-1P15nh31C-Xa4vbhPlnHApm-u7hIrf5fhQXsuvH1by26d1Be8uVvI9-QNgPapO</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1667878671</pqid></control><display><type>article</type><title>Synthesis, characterization and in vitro evaluation of cytotoxicity and antimicrobial activity of chitosan-metal nanocomposites</title><source>Wiley-Blackwell Read & Publish Collection</source><creator>Kaur, Pawan ; Thakur, Rajesh ; Barnela, Manju ; Chopra, Meenu ; Manuja, Anju ; Chaudhury, Ashok</creator><creatorcontrib>Kaur, Pawan ; Thakur, Rajesh ; Barnela, Manju ; Chopra, Meenu ; Manuja, Anju ; Chaudhury, Ashok</creatorcontrib><description>BACKGROUND
The present study explores the synthesis of chitosan–metal nanocomposites in view of their increasing application as antimicrobial material.
RESULTS
Chitosan nanoparticles were prepared by ionic gelation between chitosan and sodium tripolyphosphate. Copper sulphate hydrate (CuSO4.5H2O) and zinc acetate (Zn (O2CCH3)2) were used as precursors for synthesis of chitosan–copper nanocomposites (Cu/Ch) and chitosan–zinc nanocomposites (Zn/Ch), respectively. Synthesis of nanocomposites was confirmed by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy with energy dispersive X‐ray microanalyses (SEM‐EDX) and differential scanning calorimetry (DSC). Cytotoxicity of nanoformulations was studied by Resazurin assay on Vero cell line (African green monkey kidney cell line). Their antibacterial activities were assessed by the zone of inhibition method and time dependent growth curve against Micrococcus luteus MTCC 1809, Pseudomonas aeruginosa MTCC 424 and Salmonella enterica MTCC 1253 in vitro. Antifungal activity was also studied against Alternaria alterneta, Rhizoctonia solani and Aspergillus flavus in vitro by the mycelium inhibition method.
CONCLUSIONS
It was observed that all nanoformulations show high antimicrobial activity against all test microorganisms. So chitosan–metal complexes could be promising candidates for novel antimicrobial agents in cosmetic, food and textile industries. © 2014 Society of Chemical Industry</description><identifier>ISSN: 0268-2575</identifier><identifier>EISSN: 1097-4660</identifier><identifier>DOI: 10.1002/jctb.4383</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Alternaria ; Antiinfectives and antibacterials ; antimicrobial ; Aspergillus flavus ; Biotechnology ; chitosan ; cytotoxicity ; Differential scanning calorimetry ; In vitro testing ; Inhibition ; Micrococcus luteus ; Nanocomposites ; percentage inhibition ; Pseudomonas aeruginosa ; Rhizoctonia solani ; Salmonella enterica ; Scanning electron microscopy ; Synthesis</subject><ispartof>Journal of chemical technology and biotechnology (1986), 2015-05, Vol.90 (5), p.867-873</ispartof><rights>2014 Society of Chemical Industry</rights><rights>2015 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5083-e6e3f5427b5bf724d6bd8f1b9f094fd3973175004147f13b4630f25ac828c1103</citedby><cites>FETCH-LOGICAL-c5083-e6e3f5427b5bf724d6bd8f1b9f094fd3973175004147f13b4630f25ac828c1103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Kaur, Pawan</creatorcontrib><creatorcontrib>Thakur, Rajesh</creatorcontrib><creatorcontrib>Barnela, Manju</creatorcontrib><creatorcontrib>Chopra, Meenu</creatorcontrib><creatorcontrib>Manuja, Anju</creatorcontrib><creatorcontrib>Chaudhury, Ashok</creatorcontrib><title>Synthesis, characterization and in vitro evaluation of cytotoxicity and antimicrobial activity of chitosan-metal nanocomposites</title><title>Journal of chemical technology and biotechnology (1986)</title><addtitle>J. Chem. Technol. Biotechnol</addtitle><description>BACKGROUND
The present study explores the synthesis of chitosan–metal nanocomposites in view of their increasing application as antimicrobial material.
RESULTS
Chitosan nanoparticles were prepared by ionic gelation between chitosan and sodium tripolyphosphate. Copper sulphate hydrate (CuSO4.5H2O) and zinc acetate (Zn (O2CCH3)2) were used as precursors for synthesis of chitosan–copper nanocomposites (Cu/Ch) and chitosan–zinc nanocomposites (Zn/Ch), respectively. Synthesis of nanocomposites was confirmed by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy with energy dispersive X‐ray microanalyses (SEM‐EDX) and differential scanning calorimetry (DSC). Cytotoxicity of nanoformulations was studied by Resazurin assay on Vero cell line (African green monkey kidney cell line). Their antibacterial activities were assessed by the zone of inhibition method and time dependent growth curve against Micrococcus luteus MTCC 1809, Pseudomonas aeruginosa MTCC 424 and Salmonella enterica MTCC 1253 in vitro. Antifungal activity was also studied against Alternaria alterneta, Rhizoctonia solani and Aspergillus flavus in vitro by the mycelium inhibition method.
CONCLUSIONS
It was observed that all nanoformulations show high antimicrobial activity against all test microorganisms. So chitosan–metal complexes could be promising candidates for novel antimicrobial agents in cosmetic, food and textile industries. © 2014 Society of Chemical Industry</description><subject>Alternaria</subject><subject>Antiinfectives and antibacterials</subject><subject>antimicrobial</subject><subject>Aspergillus flavus</subject><subject>Biotechnology</subject><subject>chitosan</subject><subject>cytotoxicity</subject><subject>Differential scanning calorimetry</subject><subject>In vitro testing</subject><subject>Inhibition</subject><subject>Micrococcus luteus</subject><subject>Nanocomposites</subject><subject>percentage inhibition</subject><subject>Pseudomonas aeruginosa</subject><subject>Rhizoctonia solani</subject><subject>Salmonella enterica</subject><subject>Scanning electron microscopy</subject><subject>Synthesis</subject><issn>0268-2575</issn><issn>1097-4660</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqN0c-L1DAUB_AgCo6rB_-DgheF7e5Lkybp0R3c8ceqiCN6C2kmYTK2yZhkxq0X_3Xb7bIHQfAUyPt8H3l5CD3FcIYBqvOdzu0ZJYLcQwsMDS8pY3AfLaBioqxqXj9Ej1LaAQATFVug358Hn7cmuXRa6K2KSmcT3S-VXfCF8pvC-eLocgyFOaruMN8HW-ghhxyunXZ5uHHKZ9c7HUPrVFeMbdxxKk1063JIype9yWPJKx906PchuWzSY_TAqi6ZJ7fnCfpy-Wq9fF1efVy9Wb68KnUNgpSGGWJrWvG2bi2v6Ia1G2Fx21hoqN2QhhPMawCKKbeYtJQRsFWttKiExhjICXo-993H8ONgUpa9S9p0nfImHJLEjPMGOBf_RQkRVFA80md_0V04RD8OMirGBReMT-rFrMbfSSkaK_fR9SoOEoOctianrclpa6M9n-1P15nh31C-Xa4vbhPlnHApm-u7hIrf5fhQXsuvH1by26d1Be8uVvI9-QNgPapO</recordid><startdate>201505</startdate><enddate>201505</enddate><creator>Kaur, Pawan</creator><creator>Thakur, Rajesh</creator><creator>Barnela, Manju</creator><creator>Chopra, Meenu</creator><creator>Manuja, Anju</creator><creator>Chaudhury, Ashok</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7QL</scope><scope>7SU</scope></search><sort><creationdate>201505</creationdate><title>Synthesis, characterization and in vitro evaluation of cytotoxicity and antimicrobial activity of chitosan-metal nanocomposites</title><author>Kaur, Pawan ; Thakur, Rajesh ; Barnela, Manju ; Chopra, Meenu ; Manuja, Anju ; Chaudhury, Ashok</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5083-e6e3f5427b5bf724d6bd8f1b9f094fd3973175004147f13b4630f25ac828c1103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Alternaria</topic><topic>Antiinfectives and antibacterials</topic><topic>antimicrobial</topic><topic>Aspergillus flavus</topic><topic>Biotechnology</topic><topic>chitosan</topic><topic>cytotoxicity</topic><topic>Differential scanning calorimetry</topic><topic>In vitro testing</topic><topic>Inhibition</topic><topic>Micrococcus luteus</topic><topic>Nanocomposites</topic><topic>percentage inhibition</topic><topic>Pseudomonas aeruginosa</topic><topic>Rhizoctonia solani</topic><topic>Salmonella enterica</topic><topic>Scanning electron microscopy</topic><topic>Synthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaur, Pawan</creatorcontrib><creatorcontrib>Thakur, Rajesh</creatorcontrib><creatorcontrib>Barnela, Manju</creatorcontrib><creatorcontrib>Chopra, Meenu</creatorcontrib><creatorcontrib>Manuja, Anju</creatorcontrib><creatorcontrib>Chaudhury, Ashok</creatorcontrib><collection>Istex</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Engineering Abstracts</collection><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaur, Pawan</au><au>Thakur, Rajesh</au><au>Barnela, Manju</au><au>Chopra, Meenu</au><au>Manuja, Anju</au><au>Chaudhury, Ashok</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis, characterization and in vitro evaluation of cytotoxicity and antimicrobial activity of chitosan-metal nanocomposites</atitle><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle><addtitle>J. Chem. Technol. Biotechnol</addtitle><date>2015-05</date><risdate>2015</risdate><volume>90</volume><issue>5</issue><spage>867</spage><epage>873</epage><pages>867-873</pages><issn>0268-2575</issn><eissn>1097-4660</eissn><abstract>BACKGROUND
The present study explores the synthesis of chitosan–metal nanocomposites in view of their increasing application as antimicrobial material.
RESULTS
Chitosan nanoparticles were prepared by ionic gelation between chitosan and sodium tripolyphosphate. Copper sulphate hydrate (CuSO4.5H2O) and zinc acetate (Zn (O2CCH3)2) were used as precursors for synthesis of chitosan–copper nanocomposites (Cu/Ch) and chitosan–zinc nanocomposites (Zn/Ch), respectively. Synthesis of nanocomposites was confirmed by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy with energy dispersive X‐ray microanalyses (SEM‐EDX) and differential scanning calorimetry (DSC). Cytotoxicity of nanoformulations was studied by Resazurin assay on Vero cell line (African green monkey kidney cell line). Their antibacterial activities were assessed by the zone of inhibition method and time dependent growth curve against Micrococcus luteus MTCC 1809, Pseudomonas aeruginosa MTCC 424 and Salmonella enterica MTCC 1253 in vitro. Antifungal activity was also studied against Alternaria alterneta, Rhizoctonia solani and Aspergillus flavus in vitro by the mycelium inhibition method.
CONCLUSIONS
It was observed that all nanoformulations show high antimicrobial activity against all test microorganisms. So chitosan–metal complexes could be promising candidates for novel antimicrobial agents in cosmetic, food and textile industries. © 2014 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/jctb.4383</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0268-2575 |
| ispartof | Journal of chemical technology and biotechnology (1986), 2015-05, Vol.90 (5), p.867-873 |
| issn | 0268-2575 1097-4660 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1677907780 |
| source | Wiley-Blackwell Read & Publish Collection |
| subjects | Alternaria Antiinfectives and antibacterials antimicrobial Aspergillus flavus Biotechnology chitosan cytotoxicity Differential scanning calorimetry In vitro testing Inhibition Micrococcus luteus Nanocomposites percentage inhibition Pseudomonas aeruginosa Rhizoctonia solani Salmonella enterica Scanning electron microscopy Synthesis |
| title | Synthesis, characterization and in vitro evaluation of cytotoxicity and antimicrobial activity of chitosan-metal nanocomposites |
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