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Transformation of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita
Acremonium implicatum is an endophytic fungus with biocontrol potential against Meloidogyne incognita based on its opportunistic egg-parasitic, hatching inhibition, and toxic properties. To understand its mode of plant endophytism and opportunistic egg parasitism, GFP-tagged A. implicatum was constr...
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| Published in: | World journal of microbiology & biotechnology 2015-04, Vol.31 (4), p.549-556 |
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| Main Authors: | , , , , , |
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| cited_by | cdi_FETCH-LOGICAL-c499t-98da9f374ac16620f05a86a7aa15dfa209f4bafa05a0037f0fb8288897d7d6e23 |
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| cites | cdi_FETCH-LOGICAL-c499t-98da9f374ac16620f05a86a7aa15dfa209f4bafa05a0037f0fb8288897d7d6e23 |
| container_end_page | 556 |
| container_issue | 4 |
| container_start_page | 549 |
| container_title | World journal of microbiology & biotechnology |
| container_volume | 31 |
| creator | Yao, Yu-Rong Tian, Xue-Liang Shen, Bao-Ming Mao, Zhen-Chuan Chen, Guo-hua Xie, Bing-Yan |
| description | Acremonium implicatum is an endophytic fungus with biocontrol potential against Meloidogyne incognita based on its opportunistic egg-parasitic, hatching inhibition, and toxic properties. To understand its mode of plant endophytism and opportunistic egg parasitism, GFP-tagged A. implicatum was constructed by PEG-mediated protoplast transformation. By laser scanning confocal microscopy (LSCM), we evaluated the endophytism and opportunistic egg parasitism of a stable gfp transformant (Acr-1). Acr-1 could colonize epidermal tissue, cortical tissue, and xylem of roots and form a mutualistic symbiosis with tomato host plants. LSCM of Acr-1 infecting M. incognita eggs revealed that hyphae penetrated the shell and grew inside eggs to form trophic hyphae. A large number of hyphae enveloped parasitized eggs. In addition, the egg shell integrity was destroyed by fungal penetration. The percentage of egg parasitism was 33.8 %. There were no marked differences between the wild type and mutant in nematode second-stage juvenile mortality and egg hatching and in fungal control efficiency in a pot experiment. In conclusion, gfp-transformation did not change the nematicidal activity of A. implicatum and is a tool to examine the mode of plant endophytism and opportunistic egg parasitism of A. implicatum. |
| doi_str_mv | 10.1007/s11274-014-1781-2 |
| format | article |
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To understand its mode of plant endophytism and opportunistic egg parasitism, GFP-tagged A. implicatum was constructed by PEG-mediated protoplast transformation. By laser scanning confocal microscopy (LSCM), we evaluated the endophytism and opportunistic egg parasitism of a stable gfp transformant (Acr-1). Acr-1 could colonize epidermal tissue, cortical tissue, and xylem of roots and form a mutualistic symbiosis with tomato host plants. LSCM of Acr-1 infecting M. incognita eggs revealed that hyphae penetrated the shell and grew inside eggs to form trophic hyphae. A large number of hyphae enveloped parasitized eggs. In addition, the egg shell integrity was destroyed by fungal penetration. The percentage of egg parasitism was 33.8 %. There were no marked differences between the wild type and mutant in nematode second-stage juvenile mortality and egg hatching and in fungal control efficiency in a pot experiment. In conclusion, gfp-transformation did not change the nematicidal activity of A. implicatum and is a tool to examine the mode of plant endophytism and opportunistic egg parasitism of A. implicatum.</description><identifier>ISSN: 0959-3993</identifier><identifier>EISSN: 1573-0972</identifier><identifier>DOI: 10.1007/s11274-014-1781-2</identifier><identifier>PMID: 25724297</identifier><language>eng</language><publisher>Dordrecht: Springer-Verlag</publisher><subject>Acremonium ; Acremonium - genetics ; Acremonium - growth & development ; Acremonium - physiology ; Analysis ; Animals ; Antibiosis ; Applied Microbiology ; Biochemistry ; Biological control ; Biomedical and Life Sciences ; Biotechnology ; Confocal ; confocal laser scanning microscopy ; egg shell ; Eggs ; endophytes ; Endophytes - genetics ; Endophytes - growth & development ; Endophytes - physiology ; Environmental Engineering/Biotechnology ; Enzymes ; Experiments ; Fungi ; Green Fluorescent Proteins - genetics ; Green Fluorescent Proteins - metabolism ; Hatching ; Host plants ; hyphae ; Hyphae - genetics ; Hyphae - growth & development ; Hyphae - physiology ; Life Sciences ; Lycopersicon esculentum ; Lycopersicon esculentum - parasitology ; Meloidogyne incognita ; Microbiology ; Microscopy ; mortality ; mutants ; nematicidal properties ; Nematoda ; Nematodes ; Original Paper ; Parasitism ; Parasitology ; Pest Control, Biological - methods ; Plant Diseases - parasitology ; Plant tissues ; Plants (organisms) ; protoplasts ; roots ; Shells ; Studies ; Symbiosis ; Tomatoes ; toxicity ; Transformation, Genetic ; Transformations ; Tylenchoidea - microbiology ; Tylenchoidea - physiology ; Worms ; xylem</subject><ispartof>World journal of microbiology & biotechnology, 2015-04, Vol.31 (4), p.549-556</ispartof><rights>Springer Science+Business Media Dordrecht 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c499t-98da9f374ac16620f05a86a7aa15dfa209f4bafa05a0037f0fb8288897d7d6e23</citedby><cites>FETCH-LOGICAL-c499t-98da9f374ac16620f05a86a7aa15dfa209f4bafa05a0037f0fb8288897d7d6e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1663406814/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$H</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1663406814?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,776,780,11668,27903,27904,36039,36040,44342,74641</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25724297$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yao, Yu-Rong</creatorcontrib><creatorcontrib>Tian, Xue-Liang</creatorcontrib><creatorcontrib>Shen, Bao-Ming</creatorcontrib><creatorcontrib>Mao, Zhen-Chuan</creatorcontrib><creatorcontrib>Chen, Guo-hua</creatorcontrib><creatorcontrib>Xie, Bing-Yan</creatorcontrib><title>Transformation of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita</title><title>World journal of microbiology & biotechnology</title><addtitle>World J Microbiol Biotechnol</addtitle><addtitle>World J Microbiol Biotechnol</addtitle><description>Acremonium implicatum is an endophytic fungus with biocontrol potential against Meloidogyne incognita based on its opportunistic egg-parasitic, hatching inhibition, and toxic properties. To understand its mode of plant endophytism and opportunistic egg parasitism, GFP-tagged A. implicatum was constructed by PEG-mediated protoplast transformation. By laser scanning confocal microscopy (LSCM), we evaluated the endophytism and opportunistic egg parasitism of a stable gfp transformant (Acr-1). Acr-1 could colonize epidermal tissue, cortical tissue, and xylem of roots and form a mutualistic symbiosis with tomato host plants. LSCM of Acr-1 infecting M. incognita eggs revealed that hyphae penetrated the shell and grew inside eggs to form trophic hyphae. A large number of hyphae enveloped parasitized eggs. In addition, the egg shell integrity was destroyed by fungal penetration. The percentage of egg parasitism was 33.8 %. There were no marked differences between the wild type and mutant in nematode second-stage juvenile mortality and egg hatching and in fungal control efficiency in a pot experiment. In conclusion, gfp-transformation did not change the nematicidal activity of A. implicatum and is a tool to examine the mode of plant endophytism and opportunistic egg parasitism of A. implicatum.</description><subject>Acremonium</subject><subject>Acremonium - genetics</subject><subject>Acremonium - growth & development</subject><subject>Acremonium - physiology</subject><subject>Analysis</subject><subject>Animals</subject><subject>Antibiosis</subject><subject>Applied Microbiology</subject><subject>Biochemistry</subject><subject>Biological control</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Confocal</subject><subject>confocal laser scanning microscopy</subject><subject>egg shell</subject><subject>Eggs</subject><subject>endophytes</subject><subject>Endophytes - genetics</subject><subject>Endophytes - growth & development</subject><subject>Endophytes - physiology</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Enzymes</subject><subject>Experiments</subject><subject>Fungi</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Hatching</subject><subject>Host plants</subject><subject>hyphae</subject><subject>Hyphae - genetics</subject><subject>Hyphae - growth & development</subject><subject>Hyphae - physiology</subject><subject>Life Sciences</subject><subject>Lycopersicon esculentum</subject><subject>Lycopersicon esculentum - parasitology</subject><subject>Meloidogyne incognita</subject><subject>Microbiology</subject><subject>Microscopy</subject><subject>mortality</subject><subject>mutants</subject><subject>nematicidal properties</subject><subject>Nematoda</subject><subject>Nematodes</subject><subject>Original Paper</subject><subject>Parasitism</subject><subject>Parasitology</subject><subject>Pest Control, Biological - methods</subject><subject>Plant Diseases - parasitology</subject><subject>Plant tissues</subject><subject>Plants (organisms)</subject><subject>protoplasts</subject><subject>roots</subject><subject>Shells</subject><subject>Studies</subject><subject>Symbiosis</subject><subject>Tomatoes</subject><subject>toxicity</subject><subject>Transformation, Genetic</subject><subject>Transformations</subject><subject>Tylenchoidea - microbiology</subject><subject>Tylenchoidea - 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of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita</title><author>Yao, Yu-Rong ; Tian, Xue-Liang ; Shen, Bao-Ming ; Mao, Zhen-Chuan ; Chen, Guo-hua ; Xie, Bing-Yan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c499t-98da9f374ac16620f05a86a7aa15dfa209f4bafa05a0037f0fb8288897d7d6e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acremonium</topic><topic>Acremonium - genetics</topic><topic>Acremonium - growth & development</topic><topic>Acremonium - physiology</topic><topic>Analysis</topic><topic>Animals</topic><topic>Antibiosis</topic><topic>Applied Microbiology</topic><topic>Biochemistry</topic><topic>Biological control</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Confocal</topic><topic>confocal laser scanning microscopy</topic><topic>egg 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biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yao, Yu-Rong</au><au>Tian, Xue-Liang</au><au>Shen, Bao-Ming</au><au>Mao, Zhen-Chuan</au><au>Chen, Guo-hua</au><au>Xie, Bing-Yan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transformation of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita</atitle><jtitle>World journal of microbiology & biotechnology</jtitle><stitle>World J Microbiol Biotechnol</stitle><addtitle>World J Microbiol Biotechnol</addtitle><date>2015-04-01</date><risdate>2015</risdate><volume>31</volume><issue>4</issue><spage>549</spage><epage>556</epage><pages>549-556</pages><issn>0959-3993</issn><eissn>1573-0972</eissn><abstract>Acremonium implicatum is an endophytic fungus with biocontrol potential against Meloidogyne incognita based on its opportunistic egg-parasitic, hatching inhibition, and toxic properties. To understand its mode of plant endophytism and opportunistic egg parasitism, GFP-tagged A. implicatum was constructed by PEG-mediated protoplast transformation. By laser scanning confocal microscopy (LSCM), we evaluated the endophytism and opportunistic egg parasitism of a stable gfp transformant (Acr-1). Acr-1 could colonize epidermal tissue, cortical tissue, and xylem of roots and form a mutualistic symbiosis with tomato host plants. LSCM of Acr-1 infecting M. incognita eggs revealed that hyphae penetrated the shell and grew inside eggs to form trophic hyphae. A large number of hyphae enveloped parasitized eggs. In addition, the egg shell integrity was destroyed by fungal penetration. The percentage of egg parasitism was 33.8 %. There were no marked differences between the wild type and mutant in nematode second-stage juvenile mortality and egg hatching and in fungal control efficiency in a pot experiment. In conclusion, gfp-transformation did not change the nematicidal activity of A. implicatum and is a tool to examine the mode of plant endophytism and opportunistic egg parasitism of A. implicatum.</abstract><cop>Dordrecht</cop><pub>Springer-Verlag</pub><pmid>25724297</pmid><doi>10.1007/s11274-014-1781-2</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0959-3993 |
| ispartof | World journal of microbiology & biotechnology, 2015-04, Vol.31 (4), p.549-556 |
| issn | 0959-3993 1573-0972 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1677910507 |
| source | ABI/INFORM Global (ProQuest); Springer Nature |
| subjects | Acremonium Acremonium - genetics Acremonium - growth & development Acremonium - physiology Analysis Animals Antibiosis Applied Microbiology Biochemistry Biological control Biomedical and Life Sciences Biotechnology Confocal confocal laser scanning microscopy egg shell Eggs endophytes Endophytes - genetics Endophytes - growth & development Endophytes - physiology Environmental Engineering/Biotechnology Enzymes Experiments Fungi Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Hatching Host plants hyphae Hyphae - genetics Hyphae - growth & development Hyphae - physiology Life Sciences Lycopersicon esculentum Lycopersicon esculentum - parasitology Meloidogyne incognita Microbiology Microscopy mortality mutants nematicidal properties Nematoda Nematodes Original Paper Parasitism Parasitology Pest Control, Biological - methods Plant Diseases - parasitology Plant tissues Plants (organisms) protoplasts roots Shells Studies Symbiosis Tomatoes toxicity Transformation, Genetic Transformations Tylenchoidea - microbiology Tylenchoidea - physiology Worms xylem |
| title | Transformation of the endophytic fungus Acremonium implicatum with GFP and evaluation of its biocontrol effect against Meloidogyne incognita |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T13%3A38%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Transformation%20of%20the%20endophytic%20fungus%20Acremonium%20implicatum%20with%20GFP%20and%20evaluation%20of%20its%20biocontrol%20effect%20against%20Meloidogyne%20incognita&rft.jtitle=World%20journal%20of%20microbiology%20&%20biotechnology&rft.au=Yao,%20Yu-Rong&rft.date=2015-04-01&rft.volume=31&rft.issue=4&rft.spage=549&rft.epage=556&rft.pages=549-556&rft.issn=0959-3993&rft.eissn=1573-0972&rft_id=info:doi/10.1007/s11274-014-1781-2&rft_dat=%3Cproquest_cross%3E1677910507%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c499t-98da9f374ac16620f05a86a7aa15dfa209f4bafa05a0037f0fb8288897d7d6e23%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1663406814&rft_id=info:pmid/25724297&rfr_iscdi=true |