Influence of red algal sulfated polysaccharides on blood coagulation and platelets activation in vitro

• Published in: Journal of biomedical materials research. Part A 2014-05, Vol.102 (5), p.1431-1438
• Main Authors: Sokolova, Ekaterina V., Byankina, Anna O., Kalitnik, Alexandra A., Kim, Yong H., Bogdanovich, Larisa N., Solov'eva, Tamara F., Yermak, Irina M.
• Format: Article
• Language: English
• Subjects: Activation; Adhesion; Agglomeration; Algae; Animals; Anticoagulants; Blood; Blood Coagulation - drug effects; Blood Platelets - drug effects; Blood Platelets - physiology; Carrageenan; Carrageenan - pharmacology; Cattle; coagulation; Collagen; Collagen - pharmacology; Erythrocytes - drug effects; Erythrocytes - metabolism; Factor Xa - metabolism; Fibrinogen - pharmacology; Glycoproteins; Hemagglutination Tests; Hemolysis - drug effects; Heparins; Humans; Inhibitors; Molecular weight; Partial Thromboplastin Time; Platelet Activation - drug effects; platelet adhesion/aggregation; Platelet Adhesiveness - drug effects; Platelet Aggregation - drug effects; Platelets; Polysaccharides; Polysaccharides - pharmacology; porphyran; Prothrombin Time; red blood cells; Rhodophyta - chemistry; Sepharose - analogs & derivatives; Sepharose - pharmacology; Thrombin Time
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.34827

The influence of sulfated polysaccharides (λ‐, κ‐, and κ/β‐carrageenan and porphyran) – on platelet activation was studied. Carrageenans were much weaker inhibitors of a coagulation process than heparin, while porphyran had not that effect. Results of the aPTT and PT assays suppose that carrageenans affected mostly intrinsic pathway of coagulation, while their effect on the extrinsic pathway is extremely low (λ and κ/β) or absent (κ, LMW derivative of κ‐carrageenan). λ‐Carrageenan was the most potent anticoagulant agent in TT, aPTT, PT, and anti‐factor Xa activity. This sample was also the strongest inhibitor of collagen‐induced platelet aggregation in PRP. Generally, the correlation of anticoagulant and antithrombotic action in PRP is preserved for carrageenans but not for heparin. Carrageenans and porphyran affected platelet adhesion to collagen by influencing glycoprotein VI. Low molecular weight κ‐carrageenan had a similar effect on platelet adhesion mediated with both major collagen receptors: integrin α2β1 and glycoprotein VI as native polysaccharide had. Carrageenans resulted in activation of platelets under platelet adhesion mediated by integrin αIIbβ3 with less degree than heparin. The least sulfated κ/β‐carrageenan that possessed an inhibiting effect on thrombin‐ and collagen‐induced aggregation of washed platelets and on the PT test but it had no significant effect on TT was the weakest promoter of integrin αIIbβ3 mediated platelet activation. In summary, our study showed that the polysaccharide action was complex, since it depended on its molecular mass, sulfation degree, and monosaccharide contents (3,6‐anhydrogalactose). © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1431–1438, 2014.