A Methyl Methacrylate-HEMA-CL sub(n) Copolymerization Investigation: From Kinetics to Bioapplications

The radical copolymerization kinetics of methyl methacrylate (MMA) and poly--caprolactone macromonomer functionalized with a vinyl end group (HEMA-CL sub(n)) is studied using a pulsed-laser technique. The reactivity ratios for this system are near unity, while a linear relationship between k sub(p,c...

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Bibliographic Details
Published in:Macromolecular bioscience 2013-10, Vol.13 (10), p.1347-1357
Main Authors: Ferrari, Raffaele, Rooney, Thomas R, Lupi, Monica, Ubezio, Paolo, Hutchinson, Robin A, Moscatelli, Davide
Format: Article
Language:English
Subjects:
Biotechnology
Carriers
Copolymerization
Copolymers
Emulsion polymerization
Polymethyl methacrylates
Radicals
Reaction kinetics
Unity
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Summary:The radical copolymerization kinetics of methyl methacrylate (MMA) and poly--caprolactone macromonomer functionalized with a vinyl end group (HEMA-CL sub(n)) is studied using a pulsed-laser technique. The reactivity ratios for this system are near unity, while a linear relationship between k sub(p,cop), the copolymer-averaged propagation rate coefficient, and the composition of macromonomer in the feed (0-80wt% range) is determined. At 50wt% macromonomer in the feed, a 1.67 plus or minus 0.02 and 1.64 plus or minus 0.06 increase in k sub(p,cop)/k sub(p,MMA) is determined for HEMA-CL sub(3) and HEMA-CL sub(2), respectively. These macromonomers are adopted to synthesize nanoparticles (NPs) in the range of 100-150nm through batch emulsion free radical polymerization (BEP) to produce partially degradable drug delivery carriers. The produced NPs are tested in 4T1 cell line and show excellent characteristics as carriers: they do not affect cell proliferation, and a relevant number of NPs, thousands per cell, are internalized.[Imageomitted] Radical copolymerization kinetics of MMA and HEMA-CL sub(n) are studied using the PLP-SEC technique. The potential bioapplication of the system is investigated through the synthesis of copolymer NPs by emulsion polymerization. The NPs produced are tested in the 4T1 cell line and exhibit excellent characteristics as carriers, with low cytotoxicity and high uptake into cells.
ISSN:1616-5187
1616-5195
DOI:10.1002/mabi.201300152