In vitro evaluation of three-dimensional single-walled carbon nanotube composites for bone tissue engineering

• Published in: Journal of biomedical materials research. Part A 2014-11, Vol.102 (11), p.4118-4126
• Main Authors: Gupta, Ashim, Main, Benjamin J., Taylor, Brittany L., Gupta, Manu, Whitworth, Craig A., Cady, Craig, Freeman, Joseph W., El-Amin III, Saadiq F.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Biotechnology; Bone and Bones; bone tissue engineering; Bones; Cell adhesion; Cell Line; Cell Proliferation; Cell Survival; Cross-disciplinary physics: materials science; rheology; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Gene expression; Health. Pharmaceutical industry; Imaging; Industrial applications and implications. Economical aspects; Lactic Acid - chemistry; Materials science; Medical sciences; Mice; Microspheres; Miscellaneous; Morphology; Nanocomposites - chemistry; Nanoscale materials and structures: fabrication and characterization; Nanotubes; Nanotubes, Carbon - chemistry; Physics; PLAGA; Polyglycolic Acid - chemistry; Polylactic Acid-Polyglycolic Acid Copolymer; Regeneration; Single wall carbon nanotubes; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; SWCNT; SWCNT/PLAGA composites; SWCNT/PLAGA microspheres; Technology. Biomaterials. Equipments; Three dimensional; Tissue Engineering
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.35088

The purpose of this study was to develop three‐dimensional single‐walled carbon nanotube composites (SWCNT/PLAGA) using 10‐mg single‐walled carbon nanotubes (SWCNT) for bone regeneration and to determine the mechanical strength of the composites, and to evaluate the interaction of MC3T3‐E1 cells via cell adhesion, growth, survival, proliferation, and gene expression. PLAGA (polylactic‐co‐glycolic acid) and SWCNT/PLAGA microspheres and composites were fabricated, characterized, and mechanical testing was performed. MC3T3‐E1 cells were seeded and cell adhesion/morphology, growth/survival, proliferation, and gene expression analysis were performed to evaluate biocompatibility. Imaging studies demonstrated microspheres with uniform shape and smooth surfaces, and uniform incorporation of SWCNT into PLAGA matrix. The microspheres bonded in a random packing manner while maintaining spacing, thus resembling trabeculae of cancellous bone. Addition of SWCNT led to greater compressive modulus and ultimate compressive strength. Imaging studies revealed that MC3T3‐E1 cells adhered, grew/survived, and exhibited normal, nonstressed morphology on the composites. SWCNT/PLAGA composites exhibited higher cell proliferation rate and gene expression compared with PLAGA. These results demonstrate the potential of SWCNT/PLAGA composites for musculoskeletal regeneration, for bone tissue engineering, and are promising for orthopedic applications as they possess the combined effect of increased mechanical strength, cell proliferation, and gene expression. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 4118–4126, 2014.