Bacterial Rhizosphere and Endosphere Populations Associated with Grasses and Trees to be Used for Phytoremediation of Crude Oil Contaminated Soil

• Published in: Bulletin of environmental contamination and toxicology 2015-03, Vol.94 (3), p.314-320
• Main Authors: Fatima, Kaneez, Afzal, Muhammad, Imran, Asma, Khan, Qaiser M.
• Format: Article
• Language: English
• Subjects: Acacia; Acinetobacter; Aquatic Pollution; Bacillus; Bacteria; Bacteria - metabolism; Biodegradation; Biodegradation, Environmental; Bioremediation; Brachiaria mutica; Contamination; Crude oil; Degradation; Earth and Environmental Science; Ecotoxicology; Encoding; Environment; Environmental Chemistry; Environmental Health; Environmental Pollution; Grasses; Hydrocarbons; Hydrocarbons - metabolism; Leptochloa; Lolium - metabolism; Lolium perenne; Oil pollution; Petroleum - analysis; Petroleum - metabolism; Phytoremediation; Plant growth; Plant Roots - metabolism; Poaceae - metabolism; Poaceae - microbiology; Pollution; Rhizosphere; Soil (material); Soil contamination; Soil Microbiology; Soil Pollutants - analysis; Soil Pollutants - metabolism; Soil Science & Conservation; Spatial distribution; Trees; Trees - metabolism; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 0007-4861; 1432-0800
• DOI: 10.1007/s00128-015-1489-5

Different grasses and trees were tested for their growth in a crude oil contaminated soil. Three grasses, Lolium perenne , Leptochloa fusca , Brachiaria mutica, and two trees, Lecucaena leucocephala and Acacia ampliceps , were selected to investigate the diversity of hydrocarbon-degrading rhizospheric and endophytic bacteria. We found a higher number of hydrocarbon degrading bacteria associated with grasses than trees and that the endophytic bacteria were taxonomically different from rhizosphere associated bacteria showing their spatial distribution with reference to plant compartment as well as genotype. The rhizospheric soil yielded 22 (59.45 %), root interior yielded 9 (24.32 %) and shoot interior yielded 6 (16.21 %) hydrocarbon-degrading bacteria. These bacteria possessed genes encoding alkane hydroxylase and showed multiple plant growth-promoting activities. Bacillus (48.64 %) and Acinetobacter (18.91 %) were dominant genera found in this study. At 2 % crude oil concentration, all bacterial isolates exhibited 25 %–78 % oil degradation and Acinetobacter sp. strain BRSI56 degraded maximum. Our study suggests that for practical application, support of potential bacteria combined with the grasses is more effective approach than trees to remediate oil contaminated soils.