In vitro biocompatibility of Ti-45S5 bioglass nanocomposites and their scaffolds

• Published in: Journal of biomedical materials research. Part A 2014-05, Vol.102 (5), p.1316-1324
• Main Authors: Kaczmarek, M., Jurczyk, M. U., Rubis, B., Banaszak, A., Kolecka, A., Paszel, A., Jurczyk, K., Murias, M., Sikora, J., Jurczyk, M.
• Format: Article
• Language: English
• Subjects: Biocompatibility; Biocompatible Materials - pharmacology; Bioglass; Biological and medical sciences; Candida albicans; Candida albicans - drug effects; Candida albicans - growth & development; Cell Death - drug effects; Cell Line; cell proliferation; Cell Proliferation - drug effects; Cell Survival - drug effects; cell viability; Ceramics - pharmacology; Charge coupled devices; Colony Count, Microbial; Glass; Humans; In vitro testing; Materials Testing; Medical sciences; nanocomposite; Nanocomposites; Nanocomposites - chemistry; Nanocomposites - ultrastructure; Propidium - metabolism; scaffold; Scaffolds; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgical implants; Technology. Biomaterials. Equipments; Tissue Scaffolds - chemistry; Titanium; Titanium - pharmacology; Titanium base alloys
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.34808

Titanium–10 wt % 45S5 Bioglass nanocomposites and their scaffolds were prepared by mechanical alloying (MA) followed by pressing, sintering, or combination of MA and a “space‐holder” sintering process, respectively. An amorphous structure was obtained at 15 h of milling. The crystallization of the amorphous phase upon annealing led to the formation of a nanostructured Ti–10 wt % 45S5 Bioglass composite with a grain size of approximately 7 nm. The in vitro cytocompatibility of these materials was evaluated and compared with a conventional microcrystalline titanium. During the studies, established cell line of human fibroblasts CCD‐39Lu was cultured in the presence of tested materials and its survival rate, and proliferation activity were examined. Furthermore, the influence of the Ti–45S5 Bioglass nanocomposites and microcrystalline titanium was tested on the growth of Candida albicans yeast. Biocompatibility tests carried out indicate that the nanocomposite Ti–10 wt % 45S5 Bioglass scaffolds could be a possible candidate for dental implants and other medicinal applications. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1316–1324, 2014.