Prevention of doxorubicin sorptive losses in drug delivery studies using polyethylene glycol

The nonspecific sorption of hydrophobic pharmaceuticals on reaction vessel surfaces raises serious analytical challenges for their accurate quantification. Systematic error due to sorptive loss of analytes may result in significant overestimation of drug loading on nanomaterial-based Drug Delivery S...

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Bibliographic Details
Published in:RSC advances 2015-01, Vol.5 (33), p.25693-25698
Main Authors: Curry, Dennis, Scheller, Hope, Lu, Mingsheng, Mkandawire, Martin, Servos, Mark R, Cui, Shufen, Zhang, Xu, Oakes, Ken D
Format: Article
Language:English
Subjects:
Blocking
Doxorubicin
Drug delivery systems
Drugs
Mathematical analysis
Polyethylene glycol
Sorption
Systematic errors
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Summary:The nonspecific sorption of hydrophobic pharmaceuticals on reaction vessel surfaces raises serious analytical challenges for their accurate quantification. Systematic error due to sorptive loss of analytes may result in significant overestimation of drug loading on nanomaterial-based Drug Delivery Systems (DDS), leading to inaccurate determinations of dosage and DDS efficiency. We evaluated sorptive losses of doxorubicin (DOX), an effective chemotherapeutic, in polystyrene based 96-well plates, and proposed a simple but effective method to prevent the nonspecific sorption of DOX using trace concentrations of polyethylene glycol (PEG). Relative to widely used proteinaceous and surfactant surface blocking agents, PEG is effective, easy to use, and does not interfere with drug loading to the DDS.
ISSN:2046-2069
2046-2069
DOI:10.1039/c5ra01799h