Oral vaccination of mice with Tremella fuciformis yeast-like conidium cells expressing HBsAg

Tremella fuciformis yeast-like conidium (YLC) cells were transformed by co-cultivation with Agrobacterium cells harboring the hepatitis B surface antigen (HBsAg) gene construct under the control of the CaMV35S promoter. Integration of HBsAg DNA into the YLC genome was confirmed by PCR and dot-blot h...

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Bibliographic Details
Published in:Biotechnology letters 2015-03, Vol.37 (3), p.539-544
Main Authors: Shin, Dong-Il, Song, Kyu-Seon, Park, Hee-Sung
Format: Article
Language:English
Subjects:
Administration, Oral
Agrobacterium
Animals
Antibodies
Antigens
Applied Microbiology
Basidiomycota - genetics
Biochemistry
Biomedical and Life Sciences
Biotechnology
Boosters
coculture
DNA
Drug Carriers - administration & dosage
Drug delivery systems
Fungi
Genes
Genetic Vectors
Hepatitis
Hepatitis B
Hepatitis B Antibodies - blood
hepatitis B antigens
Hepatitis B Surface Antigens - genetics
Hepatitis B Surface Antigens - immunology
Hepatitis B Vaccines - administration & dosage
Hepatitis B Vaccines - genetics
Hepatitis B Vaccines - immunology
Hepatitis B virus
immunoblotting
Life Sciences
Mice
Microbiology
nucleic acid hybridization
oral administration
oral vaccination
Original Research Paper
polymerase chain reaction
Recombinant
Recombinant Proteins - genetics
Recombinant Proteins - immunology
Spores, Fungal - genetics
Strategy
Tremella fuciformis
Vaccination - methods
Vaccines
Vaccines, Synthetic - administration & dosage
Vaccines, Synthetic - genetics
Vaccines, Synthetic - immunology
Yeasts
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Summary:Tremella fuciformis yeast-like conidium (YLC) cells were transformed by co-cultivation with Agrobacterium cells harboring the hepatitis B surface antigen (HBsAg) gene construct under the control of the CaMV35S promoter. Integration of HBsAg DNA into the YLC genome was confirmed by PCR and dot-blot hybridization. Immunoblotting verified expression of the recombinant protein. Oral administration of YLC cells expressing HBsAg in mice significantly increased anti-HBsAg antibody titer levels using a double prime-boost strategy that combined parenteral and oral HBsAg boosters.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-014-1720-1