Plant-derived mAbs have effective anti-cancer activities by increasing ganglioside expression in colon cancers

• Published in: Biotechnology letters 2013-12, Vol.35 (12), p.2031-2038
• Main Authors: Ryu, Jae-Sung, Lee, Ju-Taek, Lim, Malg-Um, Hwang, Mi-Ran, Hwang, Kyung-A, Cho, Young-Ho, Lee, Jeong-Hwan, Ko, Kisung, Choo, Young-Kug
• Format: Article
• Language: English
• Subjects: Antibodies, Monoclonal - pharmacology; Anticancer properties; anticarcinogenic activity; Antineoplastic Agents - pharmacology; Apoptosis; Apoptosis - drug effects; Applied Microbiology; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cancer; Cancer therapies; caspase-3; caspase-6; caspase-8; caspase-9; Cell adhesion; Cell adhesion & migration; cell growth; Cell Line, Tumor; Cell Survival - drug effects; Colon; Colonic Neoplasms - metabolism; Colorectal cancer; Colorectal carcinoma; colorectal neoplasms; cyclin-dependent kinase; gangliosides; Gangliosides - metabolism; Gene Expression Regulation, Neoplastic - drug effects; Human; human growth; Humans; Life Sciences; Microbiology; Molecular biology; Monoclonal antibodies; Original Research Paper; Plant Proteins - pharmacology; Plants, Genetically Modified - metabolism; Proteins; Recombinant Proteins - pharmacology; tumor necrosis factor-alpha
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1007/s10529-013-1318-z

An epithelial cell adhesion molecule (EpCAM) was selectively expressed in human colorectal carcinoma. Treatment with plant-derived anti-EpCAM mAb (mAbᴾ CO17-1A) and RAW264.7 cells inhibited cell growth in the human colorectal cancer cell line SW620. In SW620 treated with mAbᴾ CO17-1A and RAW264.7 cells, expression of p53 and p21 increased, whereas the expression of G1 phase-related proteins, cyclin D1, CDK4, cyclin E, and CDK2, decreased, similar to mammalian-derived mAb (mAbᴹ) CO17-1A. Similar to mAbᴹ CO17-1A, treatment with mAbᴾ CO17-1A and RAW264.7 cell decreased the expression of anti-apoptotic protein, Bcl-2, but the expression of pro-apoptotic proteins Bax, TNF-α, caspase-3, caspase-6, caspase-8 and caspase-9, increased. Cells treated with mAbᴾ CO17-1A and RAW264.7 cells expressed metastasis-related gangliosides, GM1 and GD1a, similar to mAbᴹ CO17-1A. These results suggest that mAbᴾ CO17-1A is as effective on anti-cancer activity as mAbᴹ CO17-1A.