Plant-derived mAbs have effective anti-cancer activities by increasing ganglioside expression in colon cancers

An epithelial cell adhesion molecule (EpCAM) was selectively expressed in human colorectal carcinoma. Treatment with plant-derived anti-EpCAM mAb (mAbᴾ CO17-1A) and RAW264.7 cells inhibited cell growth in the human colorectal cancer cell line SW620. In SW620 treated with mAbᴾ CO17-1A and RAW264.7 ce...

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Published in:Biotechnology letters 2013-12, Vol.35 (12), p.2031-2038
Main Authors: Ryu, Jae-Sung, Lee, Ju-Taek, Lim, Malg-Um, Hwang, Mi-Ran, Hwang, Kyung-A, Cho, Young-Ho, Lee, Jeong-Hwan, Ko, Kisung, Choo, Young-Kug
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - pharmacology
Anticancer properties
anticarcinogenic activity
Antineoplastic Agents - pharmacology
Apoptosis
Apoptosis - drug effects
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cancer
Cancer therapies
caspase-3
caspase-6
caspase-8
caspase-9
Cell adhesion
Cell adhesion & migration
cell growth
Cell Line, Tumor
Cell Survival - drug effects
Colon
Colonic Neoplasms - metabolism
Colorectal cancer
Colorectal carcinoma
colorectal neoplasms
cyclin-dependent kinase
gangliosides
Gangliosides - metabolism
Gene Expression Regulation, Neoplastic - drug effects
Human
human growth
Humans
Life Sciences
Microbiology
Molecular biology
Monoclonal antibodies
Original Research Paper
Plant Proteins - pharmacology
Plants, Genetically Modified - metabolism
Proteins
Recombinant Proteins - pharmacology
tumor necrosis factor-alpha
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cited_by cdi_FETCH-LOGICAL-c462t-907e9575bd2f56fa4c70e2210a6cf4d51c43b31efceb69103b9b9cd32431cd3
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creator Ryu, Jae-Sung
Lee, Ju-Taek
Lim, Malg-Um
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Cho, Young-Ho
Lee, Jeong-Hwan
Ko, Kisung
Choo, Young-Kug
description An epithelial cell adhesion molecule (EpCAM) was selectively expressed in human colorectal carcinoma. Treatment with plant-derived anti-EpCAM mAb (mAbᴾ CO17-1A) and RAW264.7 cells inhibited cell growth in the human colorectal cancer cell line SW620. In SW620 treated with mAbᴾ CO17-1A and RAW264.7 cells, expression of p53 and p21 increased, whereas the expression of G1 phase-related proteins, cyclin D1, CDK4, cyclin E, and CDK2, decreased, similar to mammalian-derived mAb (mAbᴹ) CO17-1A. Similar to mAbᴹ CO17-1A, treatment with mAbᴾ CO17-1A and RAW264.7 cell decreased the expression of anti-apoptotic protein, Bcl-2, but the expression of pro-apoptotic proteins Bax, TNF-α, caspase-3, caspase-6, caspase-8 and caspase-9, increased. Cells treated with mAbᴾ CO17-1A and RAW264.7 cells expressed metastasis-related gangliosides, GM1 and GD1a, similar to mAbᴹ CO17-1A. These results suggest that mAbᴾ CO17-1A is as effective on anti-cancer activity as mAbᴹ CO17-1A.
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Treatment with plant-derived anti-EpCAM mAb (mAbᴾ CO17-1A) and RAW264.7 cells inhibited cell growth in the human colorectal cancer cell line SW620. In SW620 treated with mAbᴾ CO17-1A and RAW264.7 cells, expression of p53 and p21 increased, whereas the expression of G1 phase-related proteins, cyclin D1, CDK4, cyclin E, and CDK2, decreased, similar to mammalian-derived mAb (mAbᴹ) CO17-1A. Similar to mAbᴹ CO17-1A, treatment with mAbᴾ CO17-1A and RAW264.7 cell decreased the expression of anti-apoptotic protein, Bcl-2, but the expression of pro-apoptotic proteins Bax, TNF-α, caspase-3, caspase-6, caspase-8 and caspase-9, increased. Cells treated with mAbᴾ CO17-1A and RAW264.7 cells expressed metastasis-related gangliosides, GM1 and GD1a, similar to mAbᴹ CO17-1A. 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Treatment with plant-derived anti-EpCAM mAb (mAbᴾ CO17-1A) and RAW264.7 cells inhibited cell growth in the human colorectal cancer cell line SW620. In SW620 treated with mAbᴾ CO17-1A and RAW264.7 cells, expression of p53 and p21 increased, whereas the expression of G1 phase-related proteins, cyclin D1, CDK4, cyclin E, and CDK2, decreased, similar to mammalian-derived mAb (mAbᴹ) CO17-1A. Similar to mAbᴹ CO17-1A, treatment with mAbᴾ CO17-1A and RAW264.7 cell decreased the expression of anti-apoptotic protein, Bcl-2, but the expression of pro-apoptotic proteins Bax, TNF-α, caspase-3, caspase-6, caspase-8 and caspase-9, increased. Cells treated with mAbᴾ CO17-1A and RAW264.7 cells expressed metastasis-related gangliosides, GM1 and GD1a, similar to mAbᴹ CO17-1A. 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subjects Antibodies, Monoclonal - pharmacology
Anticancer properties
anticarcinogenic activity
Antineoplastic Agents - pharmacology
Apoptosis
Apoptosis - drug effects
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cancer
Cancer therapies
caspase-3
caspase-6
caspase-8
caspase-9
Cell adhesion
Cell adhesion & migration
cell growth
Cell Line, Tumor
Cell Survival - drug effects
Colon
Colonic Neoplasms - metabolism
Colorectal cancer
Colorectal carcinoma
colorectal neoplasms
cyclin-dependent kinase
gangliosides
Gangliosides - metabolism
Gene Expression Regulation, Neoplastic - drug effects
Human
human growth
Humans
Life Sciences
Microbiology
Molecular biology
Monoclonal antibodies
Original Research Paper
Plant Proteins - pharmacology
Plants, Genetically Modified - metabolism
Proteins
Recombinant Proteins - pharmacology
tumor necrosis factor-alpha
title Plant-derived mAbs have effective anti-cancer activities by increasing ganglioside expression in colon cancers
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