A paper strip based non-invasive glucose biosensor for salivary analysis

In our present study, we developed an optical biosensor for direct determination of salivary glucose by using immobilized glucose oxidase enzyme on filter paper strip (specific activity 1.4 U/strip) and then reacting it with synthetic glucose samples in presence of co-immobilized color pH indicator....

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Bibliographic Details
Published in:Biosensors & bioelectronics 2015-05, Vol.67, p.763-768
Main Authors: Soni, Anuradha, Jha, Sandeep Kumar
Format: Article
Language:English
Subjects:
Biosensing Techniques
Biosensors
Blood
Blood Glucose
Color
Diabetes Mellitus
Enzymes, Immobilized - chemistry
Filter paper
Glucose
Glucose - chemistry
Glucose - isolation & purification
Glucose Oxidase - chemistry
Humans
Profiling
Saliva
Saliva - chemistry
Source code
Strip
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Summary:In our present study, we developed an optical biosensor for direct determination of salivary glucose by using immobilized glucose oxidase enzyme on filter paper strip (specific activity 1.4 U/strip) and then reacting it with synthetic glucose samples in presence of co-immobilized color pH indicator. The filter paper changed color based on concentration of glucose in reaction media and hence, by scanning this color change (using RGB profiling) through an office scanner and open source image processing software (GIMP) the concentration of glucose in the reaction medium could be deduced. Once the biosensor was standardized, the synthetic glucose sample was replaced with human saliva from donors. The individual's blood glucose level at the time of obtaining saliva was also measured using an Accuchek(™) active glucometer (Roche Inc.). In this preliminary study, a correlation of nearly 0.64 was found between glucose levels in saliva and blood of healthy individuals and in diabetic patients it was nearly in the order of 0.95, thereby validating the importance of salivary analysis. The RGB profiling method obtained a detection range of 9-1350 mg/dL glucose at a response time of 45 s and LOD of 22.2 mg/dL.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2014.09.042