Large Scanning Range and Rapid AFM for Biological Cell Topography Imaging

The small spatial displacement of the piezo tube scanner limits the AFM(Atomic Force Microscopy) scanning range, especially when facing up to cell topography scanning. And the low dynamic property of normal AFM tube scanner tube restricts the imaging speed. In order to achieve large scanning range a...

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Bibliographic Details
Published in:Key engineering materials 2013-07, Vol.562-565, p.697-700
Main Authors: Chen, Dai Xie, Lin, Yun Sheng, Yin, Bo Hua, Gao, Ying Ying, Li, Han, Han, Dong
Format: Article
Language:English
Subjects:
Atomic force microscopy
Biological
Imaging
Liquids
Scanners
Scanning
Topography
Tubes
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Summary:The small spatial displacement of the piezo tube scanner limits the AFM(Atomic Force Microscopy) scanning range, especially when facing up to cell topography scanning. And the low dynamic property of normal AFM tube scanner tube restricts the imaging speed. In order to achieve large scanning range and rapid scanning motion simultaneously, a special atomic force microscopy is designed. The 100um scanning range is obtained by the new scanner which is composed of the flexure guide structure instead of peizo tube. Furthermore, a new acquiring image method is used to eliminating AFM nonlinearity error. Using this scanning system, some large biological cells are imaged in liquid environments with 30 lines per second.
ISSN:1013-9826
1662-9795
1662-9795
DOI:10.4028/www.scientific.net/KEM.562-565.697