Evaluation of Opuntia spp. derived products as antiproliferative agents in human colon cancer cell line (HT29)

During the last years, special interest has been focused on the recovery of bioactive compounds from food industry wastes in an effort to find added value applications for residues. In this work, Opuntia spp. residues from fruit juice production were explored as potential sources of natural chemothe...

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Bibliographic Details
Published in:Food research international 2013-11, Vol.54 (1), p.892-901
Main Authors: Serra, Ana Teresa, Poejo, Joana, Matias, Ana A., Bronze, Maria R., Duarte, Catarina M.M.
Format: Article
Language:English
Subjects:
adsorption
Amberlite (trademark)
Antiproliferative effect
Antiproliferatives
betacyanins
Betalains
bioactive properties
Biological and medical sciences
Biotechnology
Cancer
cell cycle
cell death
cell growth
Colon
Colorectal cancer
colorectal neoplasms
ferulic acid
Food industries
food processing wastes
Foods
fruit juices
Fundamental and applied biological sciences. Psychology
HT29 cell line
Human
humans
ingredients
isorhamnetin
Opuntia ficus-indica
Opuntia robusta
Opuntia spp
Polyphenols
Residues
value added
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Summary:During the last years, special interest has been focused on the recovery of bioactive compounds from food industry wastes in an effort to find added value applications for residues. In this work, Opuntia spp. residues from fruit juice production were explored as potential sources of natural chemotherapeutic ingredients towards colon cancer. Hydroalcoholic extraction and adsorption separation processes (with Amberlite XAD16 resin) were used to produce natural extracts from residues of Opuntia ficus-indica and Opuntia robusta and their antiproliferative effect was evaluated in human colon carcinoma HT29 cell line. Our results showed that the natural extracts efficiently inhibited cancer cell growth and induced cell cycle arrest in different checkpoints—G1, G2/M and S. The phytochemical constituents presented in the samples, namely betacyanins, flavonoids (isorhamnetin derivatives) and phenolic acids (ferulic acid) were identified as the main responsible compounds for the cell cycle arrest. Moreover, the extracts that presented the lowest effective dose values in terms of polyphenols (ED50 value
ISSN:0963-9969
1873-7145
DOI:10.1016/j.foodres.2013.08.043