Protective effect of Lygodium flexuosum (family: Lygodiaceae) against excision, incision and dead space wounds models in experimental rats

• Published in: Toxicology and industrial health 2015-03, Vol.31 (3), p.274-280
• Main Authors: Chandra, Phool, Yadav, Esha, Mani, Munesh, Ghosh, Ashoke Kumar, Sachan, Neetu
• Format: Article
• Language: English
• Subjects: Animals; Disease Models, Animal; Drug dosages; Elevated; Ferns - chemistry; Herbal medicine; Intoxication; Laboratory animals; Leaves; Liver; Lygodium; Male; Malondialdehyde; Medical research; Medicine; Medicine, Traditional; Ointments; Pharmaceutical sciences; Phytosterols - pharmacology; Phytotherapy; Plant Extracts - pharmacology; Plant Leaves - chemistry; Protective; Rats; Rats, Wistar; Skin - drug effects; Toxicity; Toxicology; Wound healing; Wound Healing - drug effects; Wounds and Injuries - drug therapy
• ISSN: 0748-2337; 1477-0393
• DOI: 10.1177/0748233712471704

Ethnopharmacological relevance: Lygodium flexuosum (Linn) Sw. is a climbing fern, and it is the sole genus in the family Lygodiaceae. It commonly grows epiphytically on moss covered tree trunks and branches as lithophytes on shady boulders along with moss. It has been reported as a traditional folkloric medicine for a variety of ailments particularly useful for carbuncles, inflammation, ulcer, various respiratory diseases, general disorders, muscle sprains and acts as panacea for wounds. However, there are no scientific reports on wound healing activity of the plant L. flexuosum (Linn) Sw. Aim of the study: To explore the protective effect of L. flexuosum against excision, incision and dead space wounds models in experimental rats. Methods: Wistar albino rats of either sex weighing between 180 and 220 g were topically treated with extract formulated in ointment using simple ointment BP as base. Ointments, 4% and 5% (w/w), were applied once daily in excision wound model. L. flexuosum ethanolic extract was given orally at a dose of 100, 200 and 400 mg/kg in incision and dead space wound healing models. Rats of standard groups were treated with 0.2% nitrofurazone ointment topically. The percentage wound contraction, epithelization time in excision wound model; breaking strength in incision wound model and wet and dry granulation weight, hydroxyproline content were measured. Results: Topical application of L. flexuosum in excision wound model increased the percentage of wound contraction, and the epithelization time was decreased. In the incision wound model, the breaking strength of wounds increased and in dead space model the weight of dry and wet granuloma of wounds and hydroxyproline was increased. Conclusively, the data of present study indicated that the leaf extract of L. flexuosum accelerated wound healing in rats and thus supports its traditional use.