Rice protein hydrolysate attenuates hydrogen peroxide induced apoptosis of myocardiocytes H9c2 through the Bcl-2/Bax pathway

Rat myocardiocytes H9c2 were pre-treated with rice protein hydrolysate and then treated with hydrogen peroxide (H2O2). The apoptosis rate and caspase-3 activity of H9c2 cells were analyzed by Flow Cytometry and Caspase Activity Detection Assay, respectively; the expressions of B cell lymphoma/leukem...

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Published in:Food research international 2012-10, Vol.48 (2), p.736-741
Main Authors: Yang, Tao, Zhu, He, Zhou, Hui, Lin, Qinlu, Li, Wenjian, Liu, Junwen
Format: Article
Language:English
Subjects:
Apoptosis
Attenuation
Bax
Bcl-2
Biological and medical sciences
Cereal and baking product industries
Flow cytometry
Food industries
Fundamental and applied biological sciences. Psychology
Hydrogen peroxide
Hydrolysates
Myocardiocytes
Pathways
Proteins
Rice
Rice protein hydrolysate
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cited_by cdi_FETCH-LOGICAL-c405t-719c36b56a16238b172c454ef7d998f6958c1e8f7d5b3722db1671e27e5e21df3
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creator Yang, Tao
Zhu, He
Zhou, Hui
Lin, Qinlu
Li, Wenjian
Liu, Junwen
description Rat myocardiocytes H9c2 were pre-treated with rice protein hydrolysate and then treated with hydrogen peroxide (H2O2). The apoptosis rate and caspase-3 activity of H9c2 cells were analyzed by Flow Cytometry and Caspase Activity Detection Assay, respectively; the expressions of B cell lymphoma/leukemia-2 (Bcl-2) and Bcl-2 associated X protein (Bax) were determined by real time-PCR and Western blot. The results showed that apoptosis of H9c2 cells was alleviated in response to the treatment of rice protein hydrolysate, potentially resulting from the up-regulation of Bcl-2 and the down-regulation of Bax. The results indicated that rice protein hydrolysate attenuated the apoptosis of myocardiocytes H9c2 involving in the Bcl-2/Bax pathway during H2O2 challenge, which suggest a potential role of rice protein hydrolysate on the healthcare of cardiovascular disease, especially acute myocardial ischemia. ► Anti-apoptotic effect of rice protein hydrolysate on H9c2 was determined. ► The apoptotic changes of H9c2 were determined. ► Rice protein hydrolysate attenuated the H9c2 apoptosis through bcl-2/bax pathway. ► Mechanism of rice protein hydrolysate against apoptosis in H9c2 was investigated.
doi_str_mv 10.1016/j.foodres.2012.06.017
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subjects Apoptosis
Attenuation
Bax
Bcl-2
Biological and medical sciences
Cereal and baking product industries
Flow cytometry
Food industries
Fundamental and applied biological sciences. Psychology
Hydrogen peroxide
Hydrolysates
Myocardiocytes
Pathways
Proteins
Rice
Rice protein hydrolysate
title Rice protein hydrolysate attenuates hydrogen peroxide induced apoptosis of myocardiocytes H9c2 through the Bcl-2/Bax pathway
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