A highly selective turn-on fluorescent probe for Al(III) based on coumarin and its application in vivo

In this study, a turn-on coumarin-based fluorescent probe, 7-hydroxy-6-[(2-hydroxy-naphthalen-1-ylmethylene)-amino]-4-methyl-chroman-2-one (CN), was developed for detecting Al(3+) in aqueous systems. The binding ratio of CN-Al(3+) complexes was determined from the Job plot and ESI-MS data to be 1 :...

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Bibliographic Details
Published in:Analyst (London) 2014-04, Vol.139 (8), p.1980-1986
Main Authors: Xiao, Hongde, Chen, Kun, Jiang, Nannan, Cui, Dandan, Yin, Gui, Wang, Jie, Wang, Ruiyong
Format: Article
Language:English
Subjects:
Aluminum - analysis
Animals
Binding
Biomedical materials
Coumarins - chemistry
Danio rerio
Fluorescent Dyes - chemistry
HeLa Cells
Humans
Hydrogen-Ion Concentration
In vivo testing
In vivo tests
Magnetic Resonance Spectroscopy
Mathematical analysis
Spectrometry, Fluorescence
Spectrometry, Mass, Electrospray Ionization
Surgical implants
Zebrafish
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Summary:In this study, a turn-on coumarin-based fluorescent probe, 7-hydroxy-6-[(2-hydroxy-naphthalen-1-ylmethylene)-amino]-4-methyl-chroman-2-one (CN), was developed for detecting Al(3+) in aqueous systems. The binding ratio of CN-Al(3+) complexes was determined from the Job plot and ESI-MS data to be 1 : 1. The binding constant (Ka) of Al(3+) binding to CN was calculated to be 9.55 × 10(4) M(-1) from a Benesi-Hildebrand plot and the detection limit was evaluated to be as low as 0.10 μM (LOD = 3σ/slope). CN could be used as an effective fluorescent probe for detecting Al(3+) in living HeLa cells. Moreover, CN could also be applied in the in vivo detection of Al(3+) in zebrafish.
ISSN:0003-2654
1364-5528
DOI:10.1039/c3an02247a