Physicochemical and biochemical characterization of transgenic papaya modified for protection against Papaya ringspot virus

• Published in: Journal of the science of food and agriculture 2014-03, Vol.94 (5), p.1034-1038
• Main Authors: Roberts, Madeen, Minott, Donna A, Pinnock, Simone, Tennant, Paula F, Jackson, Jose C
• Format: Article
• Language: English
• Subjects: acidity; Biochemistry; Capsid Proteins - analysis; Capsid Proteins - genetics; Capsid Proteins - metabolism; Carica - chemistry; Carica - genetics; Carica - growth & development; Carica - virology; Chemical Phenomena; color; Crops, Agricultural - chemistry; Crops, Agricultural - genetics; Crops, Agricultural - growth & development; Crops, Agricultural - virology; cultivars; Disease Resistance; Firmness; Food Quality; Food safety; Food, Genetically Modified - virology; Foods; Fruit - chemistry; Fruit - genetics; Fruit - growth & development; Fruit - virology; fruit pulp; Fruits; Functional Food - analysis; Functional Food - virology; Genetic engineering; Glucuronidase - analysis; Glucuronidase - genetics; Glucuronidase - metabolism; Jamaica; Kanamycin Kinase - analysis; Kanamycin Kinase - genetics; Kanamycin Kinase - metabolism; Limit of Detection; papaya; Papaya ringspot virus; Papaya ringspot virus (PRSV); Papayas; Physical chemistry; Plant Diseases - prevention & control; Plant Diseases - virology; Plant Leaves - chemistry; Plant Leaves - genetics; Plant Leaves - growth & development; Plant Leaves - virology; Plants, Genetically Modified - chemistry; Plants, Genetically Modified - genetics; Plants, Genetically Modified - growth & development; Plants, Genetically Modified - virology; Potyvirus - enzymology; Potyvirus - metabolism; Proteins; Recombinant Proteins - analysis; Recombinant Proteins - metabolism; Ripening; safety assessment; Species Specificity; Sunrise solo; transgenes; Transgenic; Transgenic plants; Viral Proteins - analysis; Viral Proteins - genetics; Viral Proteins - metabolism; Viruses
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.6374

BACKGROUND: Papaya, a nutritious tropical fruit, is consumed both in its fresh form and as a processed product worldwide. Major quality indices which include firmness, acidity, pH, colour and size, are cultivar dependent. Transgenic papayas engineered for resistance to Papaya ringspot virus were evaluated over the ripening period to address physicochemical quality attributes and food safety concerns. RESULTS: With the exception of one transgenic line, no significant differences (P > 0.05) were observed in firmness, acidity and pH. Lightness (L*) and redness (a*) of the pulps of non‐transgenic and transgenic papaya were similar but varied over the ripening period (P