Supercritical fluid extraction of the fortified residues of fluazifop-P-butyl (Fusilade II) and its major metabolite, fluazifop-P, in onions

A supercritical fluid extraction (SFE) procedure is described to isolate fluazifop-P-butyl and its major metabolite, fluazifop-P acid, directly from onions without any further cleanup procedures. A sample of onions is homogenized and freeze-dried. The dry sample is added to a SFE extraction vessel b...

Full description

Saved in:
Bibliographic Details
Published in:Journal of agricultural and food chemistry 1993-01, Vol.41 (1), p.84-88
Main Authors: Wigfield, Yuk Y, Lanouette, Monique
Format: Article
Language:English
Subjects:
Allium
Biological and medical sciences
CEBOLLA
Food industries
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
HERBICIDAS
HERBICIDE
METABOLITE
METABOLITOS
OIGNON
PESTICIDE
PLAGUICIDAS
PRESION
PRESSION
RESIDU
RESIDUOS
TEMPERATURA
TEMPERATURE
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A supercritical fluid extraction (SFE) procedure is described to isolate fluazifop-P-butyl and its major metabolite, fluazifop-P acid, directly from onions without any further cleanup procedures. A sample of onions is homogenized and freeze-dried. The dry sample is added to a SFE extraction vessel between two layers of silanized glass wool to prevent the clogging of the frits by fine particles in the sample. A modifier solvent (1 mL of methanol) is added with a pipet directly onto the sample, which is then extracted with supercritical fluid (SF) carbon dioxide at 80 degrees C and 400 atm for 10-min static followed by 60-min dynamic modes. The extract is trapped in three culture tubes connected in series, each containing methanol (3 mL). The methanol solutions are combined, evaporated, and analyzed by high-performance liquid chromatography (HPLC) with an ultraviolet (UV) detector. Alternately, the same extract may also be methylated and analyzed by a gas chromatograph (GC) with a mass selective detector (MSD). Using HPLC/UV, the average recoveries of fluazifop-P acid and the butyl ester at the fortification range 0.6-6.0 ppm are 94.4-78.3% and 92.8-77.8%, respectively, with a limit of detection (LOD) of 0.2 ppm. When the extract is methylated and determined by GC/MSD, the average recoveries at fortification range 0.06-0.6 ppm are 89.1-101.2% for the methyl ester and 96.9-100.6% for the butyl ester with a LOD of 0.02 ppm for both analytes
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00025a018