Monoclonal Antibody for Multiresidue ELISA of Benzimidazole Anthelmintics in Liver

A monoclonal antibody has been prepared that binds the major benzimidazole anthelmintic drugs, including albendazole, fenbendazole, oxfendazole, and several of their metabolites. In addition, the antibody binds methyl benzimidazolecarbamate, a metabolite and breakdown product of the pesticide benomy...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 1994-07, Vol.42 (7), p.1588-1594
Main Authors: Brandon, David L., Binder, Ronald G., Bates, Anne H., Montague, William C.
Format: Article
Language:English
Subjects:
ANTHELMINTHIQUE
ANTICORPS MONOCLONAL
ANTICUERPOS MONOCLONALES
ANTIHELMINTICOS
BENZIMIDAZOLE
BENZIMIDAZOLES
Biological and medical sciences
BOVIN
ELISA
FOIE
Food industries
Fundamental and applied biological sciences. Psychology
GANADO BOVINO
HIGADO
Meat and meat product industries
MEDICAMENT
MEDICAMENTOS
RESIDU
RESIDUOS
TEST ELISA
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Description
Summary:A monoclonal antibody has been prepared that binds the major benzimidazole anthelmintic drugs, including albendazole, fenbendazole, oxfendazole, and several of their metabolites. In addition, the antibody binds methyl benzimidazolecarbamate, a metabolite and breakdown product of the pesticide benomyl. The antibody was elicited from mice using the novel hapten methyl 5(6)-[(carboxypentyl) thio]-2-benzimidazolecarbamate and was used to develop an ELISA method that can detect multiple benzimidazole drug and pesticide residues at concentrations between 1 and 8 ppb. The ELISA provided the basis for quantification of drug residues in bovine liver using aqueous extraction. The sulfoxide and sulfone metabolites of albendazole and fenbendazole were readily extractable and quantifiable by this method. ELISA of liver tissue from cows treated with fenbendazole produced excellent agreement with the results of HPLC analysis. In bovine liver samples fortified with equal amounts of benzimidazole drug and sulfoxide and sulfone metabolites, the limits of detection were 58 ppb for the albendazole group and 120 ppb for the fenbendazole compounds. This sensitivity enables rapid identification of samples requiring residue-specific quantitative analysis. Since the ELISA method employs stable nonhazardous materials and reagents, it could be performed in the field for rapid screening of meat products for undesired residues
ISSN:0021-8561
1520-5118
DOI:10.1021/jf00043a039