Molecular phylogeny of the Pooideae (Poaceae) based on nuclear rDNA (ITS) sequences

Phylogenetic relationships of the Poaceae subfamily, Pooideae, were estimated from the sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA. The entire ITS region of 25 species belonging to 19 genera representing seven tribes was directly sequenced from polymerase chain...

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Bibliographic Details
Published in:Theoretical and applied genetics 1995-03, Vol.90 (3-4), p.389-398
Main Authors: Hsiao, C, Chatterton, N.J, Asay, K.H, Jensen, K.B. (United States Dept. of Agriculture/Agricultural Research Service, Utah State Univ., Logan (USA). Forage and Range Research Lab.)
Format: Article
Language:English
Subjects:
ADN
Biological and medical sciences
Biological evolution
FILOGENIA
Fundamental and applied biological sciences. Psychology
GENETICA MOLECULAR
Genetics of eukaryotes. Biological and molecular evolution
GENETIQUE MOLECULAIRE
Molecular phylogeny
NOMBRE CHROMOSOMIQUE
NUMERO DE CROMOSOMAS
ORYZA SATIVA
Pflanzenzuechtung
PHYLOGENIE
Poaceae
Pooideae
rDNA sequences
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
Triticeae
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Summary:Phylogenetic relationships of the Poaceae subfamily, Pooideae, were estimated from the sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA. The entire ITS region of 25 species belonging to 19 genera representing seven tribes was directly sequenced from polymerase chain reaction PCR-amplified DNA fragments. The published sequence of rice, Oryza sativa, was used as the outgroup. Sequences of these taxa were analyzed with maximum parsimony (PAUP) and the neighbor-joining distance method (NJ). Among the tribes, the Stipeae, Meliceae and Brachypodieae, all with small chromosomes and a basic number more than x = 7, diverged in succession. The Poeae, Aveneae, Bromeae and Triticeae, with large chromosomes and a basic number of x = 7, form a monophyletic clade. The Poeae and Aveneae are the sister group of the Bromeae and Triticeae. On the ITS tree, the Brachypodieae is distantly related to the Triticeae and Bromeae, which differs from the phylogenies based on restriction-site variation of cpDNA and morphological characters. The phylogenetic relationships of the seven pooid tribes inferred from the ITS sequences are highly concordant with the cytogenetic evidence that the reduction in chromosome number and the increase in chromosome size evolved only once in the pooids and pre-dated the divergence of the Poeae, Aveneae, Bromeae and Triticeae.
ISSN:0040-5752
1432-2242
DOI:10.1007/bf00221981