Design of multicomponent reagents for enzymatic assays

• Published in: Doklady. Biochemistry and biophysics 2015-03, Vol.461 (1), p.102-105
• Main Authors: Esimbekova, E. N., Lonshakova-Mukina, V. I., Bezrukikh, A. E., Kratasyuk, V. A.
• Format: Article
• Language: English
• Subjects: Animals; Benzoquinones - chemistry; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Biophysics and Molecular Biology; Cattle; Copper Sulfate - chemistry; Dithiothreitol - chemistry; Drug Design; Enzyme Assays - methods; Enzymes; Gelatin - chemistry; Gels - chemistry; Hydrolysis; Indicators and Reagents - chemical synthesis; Indicators and Reagents - chemistry; Life Sciences; Luciferases - chemistry; Luminescent Measurements - methods; Mercaptoethanol - chemistry; NAD - chemistry; Serum Albumin, Bovine - chemistry; Studies; Time Factors; Toxicity
• ISSN: 1607-6729; 1608-3091
• DOI: 10.1134/S1607672915020106

Currently, kits containing several lyophilized reagents, which are dissolved and dosed during the analytical procedure, are widely used in biochemical tests. The disadvantages of such reagents are the short shelf life of preparations after dissolution, the neces sity of dosing many solutions during analysis, the necessity to maintain the pH, temperature, and other conditions for preserving the high activity of enzymes, etc. In this study, we propose a new approach for designing reagent kits, which is based on selecting the conditions for co immobilization of various compo nents of enzyme systems in gels of natural origin with their subsequent drying.