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Evaluation of Phenolic Antioxidant Capacity in Grains of Modern and Old Durum Wheat Genotypes by the Novel QUENCHERABTS Approach
The QUENCHER ABTS (QUick, Easy, New, CHEap and Reproducible) approach for antioxidant capacity (AC) determination is based on the direct reaction of 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation with fine solid food particles. So, it may resemble the antioxidant actio...
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| Published in: | Plant foods for human nutrition (Dordrecht) 2015-06, Vol.70 (2), p.207-214 |
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| Main Authors: | , , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c3298-20578dc0d4e1b5810e254e1071a1d327a4a15716a54fd0999bbf7e64be0e19853 |
| container_end_page | 214 |
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| container_title | Plant foods for human nutrition (Dordrecht) |
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| creator | Laus, Maura N. Di Benedetto, Nilde A. Caporizzi, Rossella Tozzi, Damiana Soccio, Mario Giuzio, Luigia De Vita, Pasquale Flagella, Zina Pastore, Donato |
| description | The QUENCHER
ABTS
(QUick, Easy, New, CHEap and Reproducible) approach for antioxidant capacity (AC) determination is based on the direct reaction of 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation with fine solid food particles. So, it may resemble the antioxidant action in foods or in human gastrointestinal trait. Here, the QUENCHER approach was used to study AC of durum wheat (
Triticum durum
Desf.) grains. Firstly, it was assessed which kind of antioxidants determines QUENCHER response. This has been performed by comparing AC measured by QUENCHER
ABTS
and that measured by classical TEAC
ABTS
(Trolox equivalent antioxidant capacity) in four different extracts from whole flour of 10 durum wheat varieties containing: lipophilic, hydrophilic, insoluble-bound phenolic (IBP) and free-soluble phenolic (FSP) compounds. QUENCHER
ABTS
data were unrelated to AC of water-extractable antioxidants and weakly correlated (r = 0.405,
P
|
| doi_str_mv | 10.1007/s11130-015-0483-8 |
| format | article |
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ABTS
(QUick, Easy, New, CHEap and Reproducible) approach for antioxidant capacity (AC) determination is based on the direct reaction of 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation with fine solid food particles. So, it may resemble the antioxidant action in foods or in human gastrointestinal trait. Here, the QUENCHER approach was used to study AC of durum wheat (
Triticum durum
Desf.) grains. Firstly, it was assessed which kind of antioxidants determines QUENCHER response. This has been performed by comparing AC measured by QUENCHER
ABTS
and that measured by classical TEAC
ABTS
(Trolox equivalent antioxidant capacity) in four different extracts from whole flour of 10 durum wheat varieties containing: lipophilic, hydrophilic, insoluble-bound phenolic (IBP) and free-soluble phenolic (FSP) compounds. QUENCHER
ABTS
data were unrelated to AC of water-extractable antioxidants and weakly correlated (r = 0.405,
P
< 0.05) to AC of the lipophilic ones; on the contrary, QUENCHER
ABTS
response was mainly related to AC of IBP (r = 0.907,
P
< 0.001) and to a lesser extent of FSP extracts (r = 0.747,
P
< 0.001). Consistently, correlation was also found with the phenolic content of IBP and FSP (r = 0.760,
P
< 0.001 and r = 0.522,
P
< 0.01, respectively), thus confirming that QUENCHER
ABTS
assay mainly assesses AC due to IBP. So, this assay was used in a first screening study to compare AC of bioactive IBP of thirty-six genotypes/landraces covering a century of cultivation in Italy. Interestingly, no relevant AC difference between modern and old genotypes was found, thus suggesting that a century of plant breeding did not decrease phenol-dependent health potential in durum wheat.</description><identifier>ISSN: 0921-9668</identifier><identifier>EISSN: 1573-9104</identifier><identifier>DOI: 10.1007/s11130-015-0483-8</identifier><identifier>PMID: 25771798</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Antioxidants - analysis ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Ecology ; Flour - analysis ; Food Science ; Genotype ; Italy ; Nutrition ; Original Paper ; Phenols - analysis ; Plant Physiology ; Triticum - chemistry ; Triticum - classification ; Whole Grains - chemistry</subject><ispartof>Plant foods for human nutrition (Dordrecht), 2015-06, Vol.70 (2), p.207-214</ispartof><rights>Springer Science+Business Media New York 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3298-20578dc0d4e1b5810e254e1071a1d327a4a15716a54fd0999bbf7e64be0e19853</citedby><cites>FETCH-LOGICAL-c3298-20578dc0d4e1b5810e254e1071a1d327a4a15716a54fd0999bbf7e64be0e19853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25771798$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Laus, Maura N.</creatorcontrib><creatorcontrib>Di Benedetto, Nilde A.</creatorcontrib><creatorcontrib>Caporizzi, Rossella</creatorcontrib><creatorcontrib>Tozzi, Damiana</creatorcontrib><creatorcontrib>Soccio, Mario</creatorcontrib><creatorcontrib>Giuzio, Luigia</creatorcontrib><creatorcontrib>De Vita, Pasquale</creatorcontrib><creatorcontrib>Flagella, Zina</creatorcontrib><creatorcontrib>Pastore, Donato</creatorcontrib><title>Evaluation of Phenolic Antioxidant Capacity in Grains of Modern and Old Durum Wheat Genotypes by the Novel QUENCHERABTS Approach</title><title>Plant foods for human nutrition (Dordrecht)</title><addtitle>Plant Foods Hum Nutr</addtitle><addtitle>Plant Foods Hum Nutr</addtitle><description>The QUENCHER
ABTS
(QUick, Easy, New, CHEap and Reproducible) approach for antioxidant capacity (AC) determination is based on the direct reaction of 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation with fine solid food particles. So, it may resemble the antioxidant action in foods or in human gastrointestinal trait. Here, the QUENCHER approach was used to study AC of durum wheat (
Triticum durum
Desf.) grains. Firstly, it was assessed which kind of antioxidants determines QUENCHER response. This has been performed by comparing AC measured by QUENCHER
ABTS
and that measured by classical TEAC
ABTS
(Trolox equivalent antioxidant capacity) in four different extracts from whole flour of 10 durum wheat varieties containing: lipophilic, hydrophilic, insoluble-bound phenolic (IBP) and free-soluble phenolic (FSP) compounds. QUENCHER
ABTS
data were unrelated to AC of water-extractable antioxidants and weakly correlated (r = 0.405,
P
< 0.05) to AC of the lipophilic ones; on the contrary, QUENCHER
ABTS
response was mainly related to AC of IBP (r = 0.907,
P
< 0.001) and to a lesser extent of FSP extracts (r = 0.747,
P
< 0.001). Consistently, correlation was also found with the phenolic content of IBP and FSP (r = 0.760,
P
< 0.001 and r = 0.522,
P
< 0.01, respectively), thus confirming that QUENCHER
ABTS
assay mainly assesses AC due to IBP. So, this assay was used in a first screening study to compare AC of bioactive IBP of thirty-six genotypes/landraces covering a century of cultivation in Italy. Interestingly, no relevant AC difference between modern and old genotypes was found, thus suggesting that a century of plant breeding did not decrease phenol-dependent health potential in durum wheat.</description><subject>Antioxidants - analysis</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Ecology</subject><subject>Flour - analysis</subject><subject>Food Science</subject><subject>Genotype</subject><subject>Italy</subject><subject>Nutrition</subject><subject>Original Paper</subject><subject>Phenols - analysis</subject><subject>Plant Physiology</subject><subject>Triticum - chemistry</subject><subject>Triticum - classification</subject><subject>Whole Grains - chemistry</subject><issn>0921-9668</issn><issn>1573-9104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNp9kM1u3CAURlHUKJmmeYBuKpbduOXaxsByMp1MKuWvbaIsETbXGUcecMGOMrs-ehhN2mVXIDjfd3UPIR-BfQHGxNcIAAXLGPCMlbLI5AGZARdFpoCV78iMqRwyVVXymLyP8YmlTFXxI3KccyFAKDkjf5bPpp_M2HlHfUtv1-h83zV07tLTS2eNG-nCDKbpxi3tHF0F07m4Q6-8xeCocZbe9JZ-m8K0oQ9rNCNdpZJxO2Ck9ZaOa6TX_hl7-uN-eb24WP6cn939ovNhCN406w_ksDV9xNO384Tcny_vFhfZ5c3q-2J-mTVFrmSWMy6kbZgtEWougWHO05UJMGCLXJjSpM2hMrxsLVNK1XUrsCprZAhK8uKEfN73prG_J4yj3nSxwb43Dv0UNVRCilJWBSQU9mgTfIwBWz2EbmPCVgPTO_F6L14n8XonXsuU-fRWP9UbtP8Sf00nIN8DMX25Rwz6yU_BpZX_0_oKIUqMwQ</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Laus, Maura N.</creator><creator>Di Benedetto, Nilde A.</creator><creator>Caporizzi, Rossella</creator><creator>Tozzi, Damiana</creator><creator>Soccio, Mario</creator><creator>Giuzio, Luigia</creator><creator>De Vita, Pasquale</creator><creator>Flagella, Zina</creator><creator>Pastore, Donato</creator><general>Springer US</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150601</creationdate><title>Evaluation of Phenolic Antioxidant Capacity in Grains of Modern and Old Durum Wheat Genotypes by the Novel QUENCHERABTS Approach</title><author>Laus, Maura N. ; Di Benedetto, Nilde A. ; Caporizzi, Rossella ; Tozzi, Damiana ; Soccio, Mario ; Giuzio, Luigia ; De Vita, Pasquale ; Flagella, Zina ; Pastore, Donato</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3298-20578dc0d4e1b5810e254e1071a1d327a4a15716a54fd0999bbf7e64be0e19853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Antioxidants - analysis</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Chemistry/Food Science</topic><topic>Ecology</topic><topic>Flour - analysis</topic><topic>Food Science</topic><topic>Genotype</topic><topic>Italy</topic><topic>Nutrition</topic><topic>Original Paper</topic><topic>Phenols - analysis</topic><topic>Plant Physiology</topic><topic>Triticum - chemistry</topic><topic>Triticum - classification</topic><topic>Whole Grains - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laus, Maura N.</creatorcontrib><creatorcontrib>Di Benedetto, Nilde A.</creatorcontrib><creatorcontrib>Caporizzi, Rossella</creatorcontrib><creatorcontrib>Tozzi, Damiana</creatorcontrib><creatorcontrib>Soccio, Mario</creatorcontrib><creatorcontrib>Giuzio, Luigia</creatorcontrib><creatorcontrib>De Vita, Pasquale</creatorcontrib><creatorcontrib>Flagella, Zina</creatorcontrib><creatorcontrib>Pastore, Donato</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant foods for human nutrition (Dordrecht)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laus, Maura N.</au><au>Di Benedetto, Nilde A.</au><au>Caporizzi, Rossella</au><au>Tozzi, Damiana</au><au>Soccio, Mario</au><au>Giuzio, Luigia</au><au>De Vita, Pasquale</au><au>Flagella, Zina</au><au>Pastore, Donato</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Phenolic Antioxidant Capacity in Grains of Modern and Old Durum Wheat Genotypes by the Novel QUENCHERABTS Approach</atitle><jtitle>Plant foods for human nutrition (Dordrecht)</jtitle><stitle>Plant Foods Hum Nutr</stitle><addtitle>Plant Foods Hum Nutr</addtitle><date>2015-06-01</date><risdate>2015</risdate><volume>70</volume><issue>2</issue><spage>207</spage><epage>214</epage><pages>207-214</pages><issn>0921-9668</issn><eissn>1573-9104</eissn><abstract>The QUENCHER
ABTS
(QUick, Easy, New, CHEap and Reproducible) approach for antioxidant capacity (AC) determination is based on the direct reaction of 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation with fine solid food particles. So, it may resemble the antioxidant action in foods or in human gastrointestinal trait. Here, the QUENCHER approach was used to study AC of durum wheat (
Triticum durum
Desf.) grains. Firstly, it was assessed which kind of antioxidants determines QUENCHER response. This has been performed by comparing AC measured by QUENCHER
ABTS
and that measured by classical TEAC
ABTS
(Trolox equivalent antioxidant capacity) in four different extracts from whole flour of 10 durum wheat varieties containing: lipophilic, hydrophilic, insoluble-bound phenolic (IBP) and free-soluble phenolic (FSP) compounds. QUENCHER
ABTS
data were unrelated to AC of water-extractable antioxidants and weakly correlated (r = 0.405,
P
< 0.05) to AC of the lipophilic ones; on the contrary, QUENCHER
ABTS
response was mainly related to AC of IBP (r = 0.907,
P
< 0.001) and to a lesser extent of FSP extracts (r = 0.747,
P
< 0.001). Consistently, correlation was also found with the phenolic content of IBP and FSP (r = 0.760,
P
< 0.001 and r = 0.522,
P
< 0.01, respectively), thus confirming that QUENCHER
ABTS
assay mainly assesses AC due to IBP. So, this assay was used in a first screening study to compare AC of bioactive IBP of thirty-six genotypes/landraces covering a century of cultivation in Italy. Interestingly, no relevant AC difference between modern and old genotypes was found, thus suggesting that a century of plant breeding did not decrease phenol-dependent health potential in durum wheat.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>25771798</pmid><doi>10.1007/s11130-015-0483-8</doi><tpages>8</tpages></addata></record> |
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| ispartof | Plant foods for human nutrition (Dordrecht), 2015-06, Vol.70 (2), p.207-214 |
| issn | 0921-9668 1573-9104 |
| language | eng |
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| source | Springer Nature |
| subjects | Antioxidants - analysis Chemistry Chemistry and Materials Science Chemistry/Food Science Ecology Flour - analysis Food Science Genotype Italy Nutrition Original Paper Phenols - analysis Plant Physiology Triticum - chemistry Triticum - classification Whole Grains - chemistry |
| title | Evaluation of Phenolic Antioxidant Capacity in Grains of Modern and Old Durum Wheat Genotypes by the Novel QUENCHERABTS Approach |
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