A T-cell enhancer cooperates with NF-kappa B to yield cytokine induction of E-selectin gene transcription in endothelial cells

ELAM1 (endothelial leukocyte adhesion molecule 1, also known as E-selectin) is a highly tissue-specific adhesion molecule that is transiently and exclusively expressed on cytokine-induced endothelial cells. We have identified two proximal ELAM1 promoter elements and their DNA-binding factors that ar...

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Bibliographic Details
Published in:The Journal of biological chemistry 1992-11, Vol.267 (31), p.22385-22391
Main Authors: Hooft van Huijsduijnen, R, Whelan, J, Pescini, R, Becker-André, M, Schenk, A M, DeLamarter, J F
Format: Article
Language:English
Subjects:
Base Sequence
Cell Adhesion Molecules - genetics
Cells, Cultured
Cytokines - pharmacology
DNA-Binding Proteins - metabolism
E-Selectin
Endothelium, Vascular - physiology
Enhancer Elements, Genetic
Gene Expression Regulation - drug effects
In Vitro Techniques
Molecular Sequence Data
NF-kappa B - physiology
Oligodeoxyribonucleotides - chemistry
Promoter Regions, Genetic
RNA, Messenger - genetics
T-Lymphocytes - physiology
Transcription, Genetic - drug effects
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Summary:ELAM1 (endothelial leukocyte adhesion molecule 1, also known as E-selectin) is a highly tissue-specific adhesion molecule that is transiently and exclusively expressed on cytokine-induced endothelial cells. We have identified two proximal ELAM1 promoter elements and their DNA-binding factors that are, in addition to NF-kappa B, essential for ELAM1 transcription. Mutation of either element in promoter constructs carrying the first 383 nucleotides of the ELAM1 promoter markedly diminshed the expression of a fused chloramphenicol acetyltransferase reporter gene. Although multimers of either element failed to display enhancer activity on its own, fusion of the most upstream of these to the NF-kappa B element had a strong stimulatory effect. This site, ACATCAT, is recognized by a factor we have called NF-ELAM1. The site corresponds to NF-ELAM1's preferential binding sequence (A/T)CA(G/T)CA(G/T) as determined in a target definition assay. This element is identical to the T-cell delta A enhancer found in the T-cell receptor-alpha, -beta, and CD3 delta genes. Our results suggest that the delta A/NF-ELAM1 element can function as a modulator of NF-kappa B in endothelial cells both as well as a T-cell enhancer.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)41683-3