Comparison of inoculation methods for selection of plants resistant to Leptosphaeria maculans in Brassica napus

The relationship between ratings following four inoculation techniques and field ratings and the usefulness of these techniques for screening large populations was determined. Plants of Brassica napus ’Westar,’ ’Wesroona’, ’Topas’, ’Hanna’ and ’R8314’ were inoculated with L. maculans using four tech...

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Bibliographic Details
Published in:Canadian journal of plant science 1993-10, Vol.73 (4), p.1199-1207
Main Authors: McNabb, W.M, Berg, C.G.J. van den, Rimmer, S.R
Format: Article
Language:English
Subjects:
AGENT PATHOGENE
Agronomy. Soil science and plant productions
AMELIORATION DES PLANTES
Biological and medical sciences
BRASSICA NAPUS
CHAMPIGNON
CULTIVARS
DISEASE RESISTANCE
FITOMEJORAMIENTO
Fundamental and applied biological sciences. Psychology
FUNGI
Genetics and breeding of economic plants
HONGOS
INOCULACION
INOCULATION
INOCULATION METHODS
LEPTOSPHAERIA MACULANS
MANITOBA
ORGANISMOS PATOGENOS
PATHOGENICITY
PATHOGENS
Pest resistance
PLANT BREEDING
PLANT PATHOGENIC FUNGI
Plant pathogens
PODER PATOGENO
POUVOIR PATHOGENE
RESISTANCE AUX MALADIES
RESISTENCIA A LA ENFERMEDAD
SELECCION
SELECTION
VARIEDADES
Varietal selection. Specialized plant breeding, plant breeding aims
VARIETE
VARIETIES
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Summary:The relationship between ratings following four inoculation techniques and field ratings and the usefulness of these techniques for screening large populations was determined. Plants of Brassica napus ’Westar,’ ’Wesroona’, ’Topas’, ’Hanna’ and ’R8314’ were inoculated with L. maculans using four techniques: cotyledon inoculation with cotyledon rating, leaf inoculation with leaf and stem rating, stem inoculation with stem rating and inoculation using infested oat kernels with stem rating. Plants from each combination of cultivar and technique were self-pollinated for evaluation of disease rating in the field. The highest precision was obtained with cotyledon rating following cotyledon inoculation. The correlation coefficient between the rating of inoculated plants and the field rating of their progeny ranged from 0.50** for oat kernel inoculation to 0.72** for cotyledon inoculation. Considerable variation for field rating was associated with each rating following artificial inoculation. Selection of resistant plants using low cotyledon reaction was a more efficient technique than leaf or stem inoculation; it was rapid, required less labour and space and correlated well with the field ratings. Key words: Leptosphaeria maculans, Brassica napus, blackleg, oilseed rape, inoculation methods, canola
ISSN:0008-4220
1918-1833
DOI:10.4141/cjps93-159