Epithelial Hyperplasia of Imaginal Discs Induced by Mutations in Drosophila Tumor Suppressor Genes: Growth and Pattern Formation in Genetic Mosaics

Lethal mutations in the giant discs ( lgd) and fat ( ft) tumor suppressor genes of Drosophila cause epithelial hyperplasia in all imaginal discs. By contrast, mutations in the vestigial ( vg) gene adversely affect cell viability in the wing imaginal discs and consequently cause loss of pattern in th...

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Bibliographic Details
Published in:Developmental biology 1995-06, Vol.169 (2), p.387-398
Main Authors: Agrawal, Namita, Joshi, Sunita, Kango, Madhuri, Saha, Dibyendu, Mishra, Arati, Sinha, Pradip
Format: Article
Language:English
Subjects:
Animals
Cell Death
Cell Division
Cloning, Molecular
Drosophila - embryology
Drosophila - genetics
Drosophila melanogaster
Epithelium - pathology
Genes, Insect
Genes, Tumor Suppressor
Hyperplasia
Mesylates - toxicity
Mosaicism
Mutation
X-Rays
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Summary:Lethal mutations in the giant discs ( lgd) and fat ( ft) tumor suppressor genes of Drosophila cause epithelial hyperplasia in all imaginal discs. By contrast, mutations in the vestigial ( vg) gene adversely affect cell viability in the wing imaginal discs and consequently cause loss of pattern in the adult wings. However, combining homozygous lgd or ft mutations with homozygous vg 1 increases the size of the wing imaginal discs and partially restores the bristle pattern in the wings of pharate adults. Comparable pattern restoration in vg 1 wings is also induced by a newly isolated weak hypomorphic lgd 3 allele. Further, mosaic analysis revealed that whereas lgd clones generated by the Minute technique display abnormal differentiation, those induced in a homozygous vg 1 background exhibit autonomous restoration of wing pattern. These results suggest that pattern restoration in vg 1 wings can serve as an assay for hyperplasia induced by mutations in Drosophila tumor suppressor genes.
ISSN:0012-1606
1095-564X
DOI:10.1006/dbio.1995.1155